| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Osteocalcin, Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein, BGLAP |
| Entrez GeneID | 632 |
| WB Predicted band size | 11.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human Osteocalcin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于Osteocalcin (N-term)抗体的3篇参考文献示例(内容为模拟简化版)：
1. **"Development of a novel N-terminal osteocalcin-specific antibody for bone turnover studies"**
*Authors: Lee S, et al.*
摘要：研究团队开发了一种高特异性识别Osteocalcin N端片段(1-49氨基酸)的单克隆抗体，验证了其在ELISA和免疫组化中的应用，证明其可区分完整Osteocalcin与代谢片段，适用于骨质疏松症患者骨代谢标志物检测。
2. **"Osteocalcin N-terminal antibodies reveal hormonal regulation in glucose metabolism"**
*Authors: Ferron M, et al.*
摘要：通过N端特异性抗体研究发现，骨源性Osteocalcin的N端片段在小鼠模型中参与调控胰岛素分泌和糖代谢，揭示了骨内分泌功能的新机制，为代谢性疾病治疗提供新靶点。
3. **"Characterization of anti-N-terminal osteocalcin antibody cross-reactivity in vertebrate models"**
*Authors: Gundberg CM, et al.*
摘要：评估了多种脊椎动物(人类、小鼠、大鼠)中Osteocalcin N端抗体的交叉反应性，证实其在不同物种骨组织切片和血清检测中的保守性，为跨物种骨发育研究提供工具支持。
4. **"Clinical utility of N-terminal osteocalcin assays in monitoring anabolic therapy"**
*Authors: Eastell R, et al.*
摘要：采用新型N端特异性抗体建立化学发光检测法，证明血清N端Osteocalcin水平与甲状旁腺激素(PTH)治疗后骨形成速率显著相关，可作为骨合成代谢疗法的动态监测指标。
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注：以上为模拟文献，实际引用需查询具体数据库(如PubMed)。如需真实文献，建议使用关键词 "Osteocalcin N-terminal antibody" + "ELISA/Western blot/bone metabolism" 进行检索。
Osteocalcin (N-term) antibody specifically targets the N-terminal region of osteocalcin, a small, non-collagenous protein predominantly synthesized by osteoblasts during bone formation. Osteocalcin, also known as bone gamma-carboxyglutamic acid (Gla) protein, is a key marker of osteoblast activity and plays critical roles in bone mineralization, calcium ion homeostasis, and energy metabolism. Its structure includes three vitamin K-dependent γ-carboxylated glutamic acid residues, which facilitate binding to hydroxyapatite crystals in the bone matrix.
The N-terminal region of osteocalcin contains unique epitopes that differentiate it from other bone-related proteins, making antibodies against this domain valuable tools for studying osteoblast differentiation, bone remodeling, and pathologies such as osteoporosis, osteoarthritis, or metabolic bone disorders. Researchers utilize Osteocalcin (N-term) antibodies in techniques like immunohistochemistry (IHC), Western blotting (WB), and ELISA to detect osteocalcin expression in tissues, serum, or cell cultures.
Emerging studies also highlight osteocalcin's endocrine functions, linking it to glucose metabolism, testosterone production, and cognitive health. Antibodies targeting its N-terminal region help elucidate these roles by enabling precise localization and quantification. Cross-reactivity with osteocalcin from species like human, mouse, and rat is common, but validation for specific applications is recommended. Overall, this antibody serves as a crucial reagent in both basic bone biology research and clinical investigations of skeletal and metabolic diseases.
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