| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cdc42 effector protein 1, Binder of Rho GTPases 5, Serum protein MSE55, CDC42EP1, BORG5, CEP1, MSE55 |
| Entrez GeneID | 11135 |
| WB Predicted band size | 40.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CDC42EP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-343 amino acids from the C-terminal region of human CDC42EP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDC42EP1抗体的3篇参考文献，按文献名称、作者和摘要内容简要整理：
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1. **文献名称**：*CDC42EP1-mediated regulation of cell polarity in collective migration*
**作者**：Smith J. et al. (2018)
**摘要**：研究利用CDC42EP1特异性抗体，通过免疫荧光和Western blot技术，揭示了CDC42EP1在细胞集体迁移中调控细胞极性的作用，并发现其通过与Cdc42的互作影响丝状伪足的形成。
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2. **文献名称**：*CDC42EP1 as a biomarker in invasive breast cancer: Antibody validation and clinical correlations*
**作者**：Chen L. et al. (2020)
**摘要**：该研究通过免疫组化验证了CDC42EP1抗体在乳腺癌组织中的特异性，发现CDC42EP1高表达与肿瘤侵袭性相关，提示其可能作为乳腺癌预后标志物。
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3. **文献名称**：*Structural insights into CDC42EP1/BORG1 interaction with septin complexes*
**作者**：Wang Y. et al. (2019)
**摘要**：研究采用CDC42EP1抗体进行共沉淀实验，发现CDC42EP1通过结合septin蛋白复合物调控细胞分裂，揭示了其在细胞骨架重塑中的分子机制。
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**备注**：以上文献摘要基于典型研究方向虚构，实际文献需通过PubMed或Google Scholar检索关键词“CDC42EP1 antibody”或“CDC42EP1 function”获取。如需具体文章，建议补充实验目的(如WB/IF/IHC应用场景)以便精准推荐。
The CDC42EP1 antibody is a crucial tool for studying the CDC42 effector protein 1 (CDC42EP1), also known as BORG1 or MSE55. CDC42EP1 belongs to the Binder of Rho GTPases (BORG) family, which interacts with the small GTPase CDC42 to regulate cytoskeletal dynamics, cell polarity, and membrane protrusion formation. This protein plays a role in organizing actin filaments by recruiting effector molecules, thereby influencing cell shape, motility, and intercellular junctions. Its expression is implicated in developmental processes, immune responses, and pathological conditions, including cancer and inflammatory diseases.
The antibody specifically targets CDC42EP1. enabling detection and quantification in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers use it to explore CDC42EP1’s interaction with CDC42. its role in signaling pathways, and its impact on cellular architecture. Polyclonal or monoclonal versions are typically generated using recombinant protein immunogens, validated for specificity and cross-reactivity. Proper validation ensures reliable results across experimental models, from cell lines to tissue samples.
Studies leveraging this antibody have shed light on CDC42EP1’s involvement in disease mechanisms, such as tumor metastasis or endothelial dysfunction, highlighting its potential as a therapeutic target. Careful optimization of antibody concentration and antigen retrieval methods is essential for accurate interpretation in diverse biological contexts.
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