WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Dentin matrix acidic phosphoprotein 1, DMP-1, Dentin matrix protein 1, DMP1 |
Entrez GeneID | 1758 |
WB Predicted band size | 55.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This DMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the N-terminal region of human DMP1. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DMP1 (N-term)抗体的参考文献示例(注:以下文献为模拟示例,非真实存在):
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1. **文献名称**:*DMP1 N-terminal Antibody Characterization in Dental Pulp Development*
**作者**:Lee, J. et al.
**摘要**:本研究通过Western blot和免疫组化验证了DMP1 (N-term)抗体的特异性,发现其在牙髓干细胞分化过程中特异性识别N端结构域,揭示DMP1在牙本质矿化中的关键作用。
2. **文献名称**:*Role of DMP1 in Bone Matrix Formation: Insights from N-terminal Antibody Staining*
**作者**:Smith, R. et al.
**摘要**:利用DMP1 (N-term)抗体进行免疫荧光分析,发现DMP1在小鼠骨组织中的空间分布与骨胶原纤维组装相关,表明其N端区域对骨基质成熟至关重要。
3. **文献名称**:*DMP1 Isoforms in Cancer Metastasis: A Comparative Study Using Domain-Specific Antibodies*
**作者**:Chen, L. et al.
**摘要**:通过对比C端和N端抗体,研究发现DMP1的N端片段在某些癌症细胞中高表达,提示其可能参与肿瘤微环境中的异常矿化过程。
4. **文献名称**:*Developmental Expression of DMP1 in Zebrafish: Antibody Validation and Functional Analysis*
**作者**:Garcia, M. et al.
**摘要**:首次在斑马鱼模型中验证DMP1 (N-term)抗体的跨物种反应性,揭示DMP1在早期骨骼发育中的保守功能,并确认抗体适用于体内免疫沉淀实验。
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如需真实文献,建议通过PubMed或Google Scholar检索关键词“DMP1 N-terminal antibody”,并筛选近年研究。
The DMP1 (N-term) antibody is designed to target the N-terminal region of dentin matrix protein 1 (DMP1), a key extracellular matrix protein primarily expressed in mineralized tissues such as teeth and bones. DMP1 belongs to the Small Integrin-Binding Ligand N-linked Glycoprotein (SIBLING) family and plays a critical role in regulating biomineralization processes. It facilitates hydroxyapatite crystal formation, promotes osteocyte differentiation, and modulates phosphate metabolism through interactions with other proteins like FGF23. Mutations in the DMP1 gene are linked to autosomal recessive hypophosphatemic rickets, underscoring its importance in skeletal and dental health.
This antibody is widely used in research to study DMP1 expression, localization, and function in tissues. Applications include immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting (WB) to detect DMP1 in osteocytes, odontoblasts, and other mineralizing cell types. Its specificity for the N-terminal region allows researchers to distinguish full-length DMP1 from proteolytically processed fragments, which may have distinct biological roles. Studies employing this antibody have advanced understanding of DMP1’s involvement in bone remodeling, dentinogenesis, and pathological conditions like osteoporosis or renal phosphate-wasting disorders. Validation data, such as reactivity in knockout controls or peptide blocking experiments, are essential to confirm antibody specificity.
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