| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Magnesium transporter NIPA4, Ichthyin, NIPA-like protein 4, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4, NIPAL4, ICHN, NIPA4 |
| Entrez GeneID | 348938 |
| WB Predicted band size | 50.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NIPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human NIPA4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于NIPA4及其相关研究的模拟参考文献示例(请注意，以下内容为假设性生成，实际文献需通过学术数据库验证)：
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1. **文献名称**：*NIPA4 encodes a Mg²⁺ transporter essential for synaptic development and function*
**作者**：Smith J, et al.
**摘要**：本研究鉴定NIPA4为新型镁离子转运蛋白，其N端结构域对蛋白稳定性至关重要。文章描述了针对N端表位的单克隆抗体的开发，证实其在神经元突触中的定位，并发现NIPA4缺失导致突触传递异常。
2. **文献名称**：*Antibody-based profiling of NIPA4 expression in human tissues*
**作者**：Lee H, et al.
**摘要**：通过生成兔多克隆抗体(靶向NIPA4的N端1-100氨基酸)，系统性分析了NIPA4在多种组织中的表达模式，发现其在肾脏和脑组织高表达，并验证了抗体在免疫组化及Western blot中的特异性。
3. **文献名称**：*Structural insights into NIPA4 function and its implications in genetic disorders*
**作者**：Garcia R, et al.
**摘要**：利用冷冻电镜解析NIPA4蛋白结构，揭示N端结构域在镁离子结合中的作用。研究使用N-term抗体进行共沉淀实验，证明其与SLC41A1的相互作用，为相关遗传病机制提供线索。
4. **文献名称**：*NIPA4 antibody validation for neurodegenerative disease models*
**作者**：Chen L, et al.
**摘要**：评估了多种商业NIPA4抗体(包括N-term特异性抗体)在小鼠和人类脑样本中的可靠性，发现部分抗体存在交叉反应，强调验证必要性，并推荐特定克隆号用于神经退行性疾病研究。
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**注意**：以上文献为示例，实际引用时请通过PubMed、Google Scholar等平台检索真实文献(关键词：NIPA4 antibody, N-terminal epitope, magnesium transporter)。若研究较少，可扩展至NIPA家族(如NIPA1、NIPA2)或SLC41A家族的相关文献。
NIPA4 (N-term) antibody is designed to target the N-terminal region of the NIPA4 protein, a member of the NIPA (non-imprinted in Prader-Willi/Angelman) magnesium transporter family. NIPA4. also known as MAGT2 or TUSC3. is a highly conserved transmembrane protein implicated in magnesium homeostasis and cellular ion transport. It shares structural homology with other NIPA family members, featuring multiple transmembrane domains and involvement in magnesium uptake across plasma or intracellular membranes. Dysregulation of NIPA4 has been linked to neurodevelopmental disorders, cancer progression, and immune dysfunction, though its precise mechanisms remain under investigation.
The antibody is commonly used in research to study NIPA4 expression, localization, and function via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Its specificity for the N-terminal region allows detection of full-length or truncated variants, aiding in the characterization of splice isoforms or disease-associated mutations. Validated in multiple species (e.g., human, mouse), the antibody often undergoes rigorous testing, including knockout (KO) validation, to ensure minimal cross-reactivity. Researchers employ it to explore NIPA4's role in magnesium-dependent processes, synaptic plasticity, and pathologies such as X-linked intellectual disability or tumor metastasis. Its utility extends to both basic research and preclinical studies aiming to clarify NIPA4's contribution to cellular physiology and disease.
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