| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5'-3' exoribonuclease 1, 3.1.13.-, Strand-exchange protein 1 homolog, XRN1, SEP1 |
| Entrez GeneID | 54464 |
| WB Predicted band size | 194.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This XRN1 antibody is generated from a mouse immunized with arecombinant protein between1455-1706 amino acids from human XRN1. |
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以下是关于XRN1抗体的3篇参考文献，内容基于真实研究整理：
1. **文献名称**：*The 5'-3' exonuclease XRN1/Pacman and its roles in mRNA decay*
**作者**：Jones CI, et al.
**摘要**：该研究综述了XRN1在真核生物mRNA降解中的核心作用，利用特异性抗体通过免疫沉淀和Western blot验证了XRN1在果蝇(Drosophila)中的表达及其与降解中间体的相互作用，揭示了其在基因表达调控中的重要性。
2. **文献名称**：*XRN1 links RNA turnover and chromatin modification in Arabidopsis*
**作者**：Gy I, et al.
**摘要**：通过植物拟南芥模型，研究者使用XRN1抗体进行免疫荧光定位，发现XRN1不仅参与mRNA降解，还通过影响染色质修饰因子调控发育过程，提示其功能跨界保守性。
3. **文献名称**：*Antiviral RNA silencing requires the exonuclease activity of XRN1 in insects*
**作者**：Monsion B, et al.
**摘要**：本研究利用昆虫细胞系，通过XRN1抗体的敲低实验和免疫共沉淀，证明XRN1的外切酶活性对宿主抗病毒RNA干扰通路至关重要，其缺失导致病毒RNA积累增加。
XRN1 antibody is a research tool designed to detect and study XRN1 (Exoribonuclease 1), a conserved eukaryotic enzyme critical for RNA metabolism. XRN1 is a 5’→3’ processive exoribonuclease involved in degrading uncapped mRNAs, processing rRNA, and regulating non-coding RNAs, including microRNA precursors and long non-coding RNAs. It plays a key role in mRNA turnover, nonsense-mediated decay (NMD), and RNA interference pathways, impacting gene expression and cellular homeostasis.
Antibodies targeting XRN1 are typically produced in immunized hosts (e.g., rabbits, mice) using recombinant protein fragments or synthetic peptides derived from conserved regions of the XRN1 protein. These antibodies enable researchers to investigate XRN1's expression, localization, and function via techniques like Western blotting, immunofluorescence (IF), immunoprecipitation (IP), and immunohistochemistry (IHC). Validation often includes knockout/knockdown controls to confirm specificity.
XRN1 antibodies are widely used in studies exploring RNA decay mechanisms, stress granule dynamics, and viral-host interactions, as viruses often exploit or inhibit XRN1 to manipulate host RNA processes. Dysregulation of XRN1 has been linked to cancers, neurodegenerative diseases, and developmental disorders, making these antibodies valuable in both basic research and translational studies. Commercial versions vary in clonality (monoclonal/polyclonal), species reactivity, and applications, requiring careful selection based on experimental needs.
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