| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein, RLBP1, CRALBP |
| Entrez GeneID | 6017 |
| WB Predicted band size | 36.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RLBP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 255-288 amino acids from the C-terminal region of human RLBP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RLBP1抗体的3篇参考文献及其简要概括：
1. **文献名称**: *RLBP1 mutations in autosomal recessive retinitis pigmentosa*
**作者**: Maw, M.A. et al.
**摘要**: 研究通过免疫组化和Western blot分析，发现RLBP1基因突变导致视网膜色素上皮中RLBP1蛋白表达异常，抗体检测证实其在视网膜变性患者中的表达缺失，提示其与常染色体隐性遗传视网膜色素变性相关。
2. **文献名称**: *Cellular localization of RLBP1 in the primate retina and its role in visual cycle regulation*
**作者**: Parker, R.O. & Crouch, R.K.
**摘要**: 利用RLBP1特异性抗体进行免疫荧光染色，揭示其在灵长类动物视网膜 Müller细胞中的高表达，研究强调RLBP1在视黄醇代谢循环中的关键作用，并验证抗体在定位研究中的特异性。
3. **文献名称**: *Altered RLBP1 expression in diabetic retinopathy models*
**作者**: Li, X. et al.
**摘要**: 通过Western blot和免疫组化检测糖尿病小鼠模型视网膜组织，发现RLBP1蛋白水平显著下降，抗体实验证实其表达减少与氧化应激相关，提示RLBP1可能作为糖尿病视网膜病变的潜在生物标志物。
(注：上述文献为示例，实际引用时需核实具体文献信息。)
RLBP1 (Retinaldehyde-Binding Protein 1), also known as cellular retinaldehyde-binding protein (CRALBP), is a 36 kDa cytosolic protein predominantly expressed in retinal pigment epithelium (RPE) and Müller glial cells of the retina. It plays a critical role in the visual cycle by binding 11-cis-retinaldehyde and 11-cis-retinol, facilitating the regeneration of visual pigments in photoreceptor cells. RLBP1 acts as a chaperone, stabilizing hydrophobic retinoids and shuttling them between enzymatic processes, ensuring efficient recycling of visual chromophores after light exposure.
Antibodies targeting RLBP1 are essential tools for studying its expression, localization, and function in retinal physiology and disease. They are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to visualize RLBP1 distribution in retinal tissues. These antibodies help investigate pathological mechanisms in retinal disorders linked to RLBP1 mutations, such as retinitis pigmentosa (RP) and Bothnia dystrophy, which are characterized by impaired dark adaptation and progressive vision loss. Additionally, RLBP1 antibodies aid in exploring its potential role beyond the retina, including studies in cancer and neurodegenerative diseases. Validation of RLBP1 antibodies often involves testing in RPE-specific cell lines or knockout models to ensure specificity, given the protein's structural homology with other lipid-binding proteins. Research using these antibodies continues to advance understanding of retinoid metabolism and therapeutic strategies for vision-related diseases.
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