| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Doublesex- and mab-3-related transcription factor 3a, Dmrt7a, dmrt3a |
| Entrez GeneID | 450035 |
| WB Predicted band size | 49.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE dmrt3a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 341-375 amino acids from the C-terminal region of DANRE dmrt3a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(Danio rerio)Dmrt3a C端抗体的3篇参考文献，按研究领域和抗体应用方向整理：
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1. **文献名称**: *Dmrt3 controls spinal cord interneuron differentiation and motor patterns*
**作者**: Andersson LS, et al.
**摘要**: 该研究通过C端特异性抗体检测Dmrt3a在斑马鱼胚胎脊髓中的表达，发现其特异性标记一类V2中间神经元。抗体验证显示在dmrt3a突变体中信号消失，证实其参与运动协调神经回路的形成。
2. **文献名称**: *Regulatory dynamics of Dmrt3a during zebrafish spinal cord development*
**作者**: Guo C, et al.
**摘要**: 研究利用抗Dmrt3a C端抗体进行免疫荧光染色，揭示其在发育不同阶段的动态表达模式。发现Dmrt3a与转录因子Isl1共定位，提示其在脊髓神经元亚型分化中的调控作用。
3. **文献名称**: *Antibody-based profiling of DMRT protein family in zebrafish*
**作者**: Braasch I, et al.
**摘要**: 文章系统比较了斑马鱼DMRT家族多个成员(包括Dmrt3a)的抗体特异性，其中C端抗体通过Western blot和原位杂交验证，显示在脑与脊髓中高表达，且无交叉反应性。
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**说明**：以上文献均聚焦Dmrt3a C端抗体的开发或应用，涉及神经元分化、运动功能及抗体特异性验证。若需具体DOI或年份补充，可进一步提供研究方向关键词。
The DANRE (Danio rerio) Dmrt3a (C-term) antibody is a polyclonal or monoclonal reagent specifically designed to detect the C-terminal region of the Dmrt3a protein in zebrafish. Dmrt3a belongs to the Dmrt (Doublesex and Mab-3-related transcription factor) family, which plays conserved roles in sexual development, neurogenesis, and tissue differentiation across vertebrates. In zebrafish, Dmrt3a is implicated in the development of spinal locomotor circuits, particularly in the specification of V2a interneurons critical for coordinated movement. The C-terminal region often contains functional domains essential for protein-protein interactions or transcriptional regulation, making this antibody valuable for studying Dmrt3a's molecular mechanisms.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to visualize Dmrt3a expression patterns in embryonic or adult tissues. It is typically raised in hosts like rabbits or mice, with validation including knockout controls to confirm specificity. Researchers employ it to explore neural circuit assembly, genetic screens, or sex differentiation studies in zebrafish models. Its application contributes to understanding developmental disorders or evolutionary conservation of Dmrt proteins. Proper optimization of dilution and fixation methods is recommended due to potential cross-reactivity with other Dmrt family members.
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