Rabbit Polyclonal (DANRE)eng2b(N-term) Antibody

The DANRE Eng2b (N-term) antibody is a specific tool designed to target the N-terminal region of the Engrailed 2b (Eng2b) protein in *Danio rerio* (zebrafish). Eng2b belongs to the Engrailed family of homeodomain-containing transcription factors, which play crucial roles in embryonic development, particularly in establishing segmental patterning and neuronal differentiation. In zebrafish, Eng2b is involved in midbrain-hindbrain boundary formation, somite development, and neural crest cell migration. The antibody is commonly used in developmental biology research to visualize Eng2b expression patterns via techniques like immunohistochemistry (IHC), immunofluorescence (IF), or Western blotting. Its specificity for the N-terminal epitope ensures minimal cross-reactivity with other Engrailed paralogs, enabling precise tracking of Eng2b dynamics during embryogenesis. Researchers employ this antibody to study gene function in neural tube formation, somitogenesis, and tissue polarity, offering insights into conserved developmental mechanisms across vertebrates. Validation typically includes knockout controls or peptide-blocking assays to confirm target specificity. Overall, the DANRE Eng2b (N-term) antibody serves as a critical reagent for dissecting the molecular basis of early vertebrate development.