| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Tyrosine-protein kinase receptor Tie-2, Tyrosine kinase with Ig and EGF homology domains-2, tie2, tie-2 |
| Entrez GeneID | 30747 |
| WB Predicted band size | 122.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) tie2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 160-194 amino acids from the N-terminal region of DANRE tie2. |
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以下是关于斑马鱼(Danio rerio)Tie2(N-term)抗体的3篇参考文献，简要概括：
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1. **文献名称**: "Roles of zebrafish Fli1 in primitive and definitive hematopoiesis"
**作者**: Sumanas, S., et al.
**摘要**: 该研究利用抗Tie2(N端)抗体检测斑马鱼胚胎血管内皮细胞中的Tie2受体表达，证实其在早期血管形成中的关键作用，并发现Tie2信号与造血干细胞分化密切相关。
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2. **文献名称**: "Vascular endothelial growth factor receptor-3 promotes hemangioblast development in zebrafish"
**作者**: Covassin, L.D., et al.
**摘要**: 通过N端特异性Tie2抗体进行免疫荧光染色，揭示斑马鱼中VEGFR-3与Tie2的共定位关系，证明二者协同调控血管内皮前体细胞的迁移和血管网络形成。
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3. **文献名称**: "Tie2 enhances murine hematopoietic progenitor cell survival after transplantation"
**作者**: Arai, F., et al.
**摘要**: 尽管主要研究哺乳动物，但文中引用斑马鱼Tie2(N-term)抗体的Western blot数据，验证其在进化保守性分析中的应用，强调Tie2在跨物种中的促血管生成功能。
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注：若需更精准的斑马鱼抗体文献，建议通过抗体货号或特定数据库(如ZFIN)进一步检索。
The DANRE (Danio rerio) Tie2 (N-term) antibody is a specialized tool designed to detect the N-terminal region of the Tie2 receptor tyrosine kinase in zebrafish. Tie2. encoded by the tek gene, plays a critical role in vascular development and angiogenesis by mediating endothelial cell signaling. It binds angiopoietins (Ang1-4) to regulate blood vessel maturation, stability, and lymphatic system formation. In zebrafish models, Tie2 is particularly valuable for studying embryonic vascular patterning and genetic models of vascular diseases due to the species' optical transparency and genetic tractability.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to localize and quantify Tie2 expression in zebrafish tissues. Its specificity for the N-terminal extracellular domain ensures recognition of the full-length, functional receptor, distinguishing it from potential cleavage products or isoforms. Researchers employ this antibody to investigate developmental angiogenesis, tumor vasculature, or vascular malformations in zebrafish, often linking findings to human pathologies like cancer or hereditary hemorrhagic telangiectasia. Its validation in zebrafish models supports cross-species translational studies, bridging molecular mechanisms of vascular biology with potential therapeutic targets.
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