| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase Nek10, Never in mitosis A-related kinase 10, NimA-related protein kinase 10, NEK10 |
| Entrez GeneID | 152110 |
| WB Predicted band size | 133.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NEK10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 450-483 amino acids from the Central region of human NEK10. |
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以下是关于NEK10抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*NEK10 regulates DNA damage response and cellular migration in breast cancer*
**作者**：Smith A, et al.
**摘要**：本研究利用NEK10特异性抗体揭示了NEK10在乳腺癌细胞中调控DNA损伤修复通路的作用，并通过免疫印迹和免疫荧光实验证明其通过磷酸化关键修复蛋白(如BRCA1)影响细胞迁移和化疗耐药性。
2. **文献名称**：*A novel monoclonal antibody targeting NEK10 for detection of lung adenocarcinoma progression*
**作者**：Chen L, et al.
**摘要**：开发了一种高灵敏度的NEK10单克隆抗体，用于肺癌组织样本的免疫组化分析，发现NEK10表达水平与肿瘤分期和转移显著相关，提示其作为潜在诊断标志物的价值。
3. **文献名称**：*NEK10 interacts with ciliogenesis proteins and modulates primary cilia formation*
**作者**：Johnson R, et al.
**摘要**：通过NEK10抗体的免疫共沉淀实验，发现NEK10与纤毛发生相关蛋白(如IFT88)相互作用，调控纤毛形成和Hedgehog信号通路，为NEK10在发育性疾病中的作用提供新机制。
4. **文献名称**：*Validation of NEK10 antibody specificity in renal cell carcinoma models*
**作者**：Wang Y, et al.
**摘要**：系统验证了商业NEK10抗体的特异性，并应用于肾癌细胞系和小鼠模型，证明NEK10通过调控细胞周期蛋白(Cyclin D1)影响肿瘤增殖，为靶向治疗提供依据。
(注：上述文献为示例，实际引用需根据具体研究检索PubMed等数据库。)
NEK10 (NIMA-related kinase 10) is a serine/threonine kinase belonging to the NEK family, which plays roles in cell cycle regulation, DNA damage response, and cilia function. It is implicated in G2/M phase progression and may participate in homologous recombination repair of DNA double-strand breaks. NEK10 has gained attention due to its potential links to cancer, particularly breast and lung cancers, where dysregulated expression or mutations are observed. Studies suggest it may act as a tumor suppressor or oncogene depending on context, making it a candidate biomarker or therapeutic target.
NEK10 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess NEK10 levels in tissues or cell lines. Commercial antibodies often target specific epitopes within its kinase domain or regulatory regions. Validation includes testing in knockout cell lines or siRNA-mediated knockdown to confirm specificity. Some antibodies also distinguish between phosphorylated (active) and inactive forms, aiding in functional studies.
Research challenges include NEK10's low abundance in certain tissues and cross-reactivity with other NEK family members. Recent studies explore its role in ciliopathies and chemoresistance, expanding its therapeutic relevance. Ongoing efforts aim to develop isoform-specific antibodies to address its splice variants. Suppliers like Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology provide NEK10 antibodies, though researchers are advised to verify performance in their experimental systems.
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