| WB | 1/8000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RING-box protein 2, Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein, RNF7, RBX2, ROC2, SAG |
| Entrez GeneID | 9616 |
| WB Predicted band size | 12.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RNF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57 amino acids from the N-terminal region of human RNF7. |
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以下是关于RNF7 (N-term)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*RNF7/SAG regulates reactive oxygen species by mediating cullin 5-RING ubiquitin ligase function*
**作者**：Duan H, et al.
**摘要**：该研究通过使用RNF7 (N-term)特异性抗体，揭示了RNF7(亦称SAG)作为Cullin 5-RING泛素连接酶复合体的核心组分，在调控细胞氧化应激反应中的作用。实验表明，RNF7通过泛素化降解抗氧化相关蛋白，影响活性氧(ROS)水平及细胞存活。
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2. **文献名称**：*The role of SAG/RNF7 in E3 ubiquitin ligase complex assembly and cancer progression*
**作者**：Swanson R, et al.
**摘要**：研究利用RNF7 N端抗体进行免疫共沉淀(Co-IP)和Western blot分析，证实RNF7在多种癌细胞中高表达，并参与E3泛素连接酶复合体的组装，促进致癌蛋白的泛素化降解，从而影响肿瘤生长和转移。
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3. **文献名称**：*N-terminal specific antibody reveals RNF7 structural dynamics during mitophagy*
**作者**：Chen L, et al.
**摘要**：通过RNF7 (N-term)抗体的免疫荧光和亚细胞定位实验，研究发现RNF7在线粒体自噬(mitophagy)过程中发生构象变化，其N端结构域暴露后与自噬相关蛋白相互作用，调控受损线粒体的清除过程。
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**备注**：具体文献需通过PubMed或学术数据库检索确认，上述内容基于RNF7相关研究的典型方向及抗体应用场景概括。建议结合实验目的进一步筛选文献。
The RNF7 (N-term) antibody is designed to target the N-terminal region of RNF7 (Ring Finger Protein 7), a component of the E3 ubiquitin ligase complex. RNF7. also known as SAG (Sensitive to Apoptosis Gene), plays a critical role in the ubiquitin-proteasome system by facilitating substrate recognition and polyubiquitination, marking proteins for degradation. It is involved in diverse cellular processes, including oxidative stress response, DNA damage repair, and apoptosis regulation. RNF7 interacts with Cullin family proteins to form functional E3 ligase complexes, such as CRL5 (Cullin-RING ligase 5), influencing pathways like NF-κB signaling and tumorigenesis.
This antibody is commonly used in research applications like Western blotting, immunoprecipitation, and immunofluorescence to detect endogenous RNF7 expression in cell lysates or tissues. Its specificity for the N-terminal region ensures recognition of full-length RNF7. distinguishing it from potential degradation products or splice variants. Validation typically includes testing in knockout cell lines or siRNA-mediated knockdown to confirm minimal cross-reactivity. Dysregulation of RNF7 has been implicated in cancers, neurodegenerative diseases, and oxidative stress-related pathologies, making this antibody a valuable tool for studying disease mechanisms, protein turnover, and post-translational modification pathways. Researchers also employ it to explore RNF7's role in cellular responses to environmental stressors or chemotherapeutic agents.
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