Rabbit Polyclonal MAP2K7 Antibody

The MAP2K7 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase 7 (MAP2K7), also known as MKK7. a dual-specificity kinase within the JNK signaling pathway. MAP2K7 phosphorylates and activates c-Jun N-terminal kinases (JNK1/2/3) in response to stress, cytokines, or growth factors, regulating processes like apoptosis, inflammation, and differentiation. Antibodies targeting MAP2K7 are widely used in Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and activation status in various tissues or cell lines. These antibodies often recognize specific epitopes, such as phosphorylated residues (e.g., Ser271/Thr275) critical for kinase activity, or non-phosphorylated regions for total protein analysis. Validation typically includes knockout/knockdown controls and species cross-reactivity checks (human, mouse, rat). Research applications span cancer (e.g., tumor progression studies), neurodegenerative diseases, and immune disorders, where dysregulated JNK signaling is implicated. MAP2K7 antibodies also aid in exploring crosstalk with other pathways (e.g., NF-κB) and screening therapeutic targets. Commercial variants may differ in clonality (monoclonal/polyclonal), conjugation (HRP, fluorescent tags), or host species (rabbit, mouse), requiring careful selection based on experimental needs. Proper controls, like phosphorylation induction via stressors (e.g., UV, TNF-α), are essential for activation-specific assays.