| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Netrin receptor UNC5B, Protein unc-5 homolog 2, Protein unc-5 homolog B, p53-regulated receptor for death and life protein 1, UNC5B, P53RDL1, UNC5H2 |
| Entrez GeneID | 219699 |
| WB Predicted band size | 103.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This UNC5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 507-541 amino acids from the Central region of human UNC5B. |
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以下是3-4条关于UNC5B抗体的参考文献及其简要摘要：
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1. **文献名称**：*UNC5B mediates G1 cell cycle arrest in endothelial cells through interaction with the cleaved intracellular domain of Robo4*
**作者**：Lu et al.
**摘要**：该研究利用UNC5B抗体进行免疫沉淀和Western blot分析，发现UNC5B通过与Robo4蛋白的胞内结构域相互作用，调控内皮细胞的G1期细胞周期停滞，揭示了其在血管生成中的新机制。
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2. **文献名称**：*Dependence receptors UNC5B trigger apoptosis in colorectal cancer cells upon ligand withdrawal*
**作者**：Mazelin et al.
**摘要**：通过免疫组化及抗体阻断实验，研究证明UNC5B在结直肠癌细胞中作为依赖性受体，在Netrin-1缺失时激活caspase通路诱导凋亡，提示其作为肿瘤抑制因子的潜力。
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3. **文献名称**：*Axon guidance receptor UNC5B regulates midline crossing in the developing spinal cord*
**作者**：Ackerman et al.
**摘要**：利用UNC5B特异性抗体进行小鼠脊髓组织染色，发现UNC5B通过排斥性信号引导轴突跨越中线，为神经发育中轴突导向的分子机制提供了证据。
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4. **文献名称**：*Proteolytic cleavage of UNC5B by caspases promotes apoptosis during neuronal development*
**作者**：Williams et al.
**摘要**：研究采用UNC5B抗体检测蛋白切割片段，发现caspase介导的UNC5B裂解增强神经元凋亡，揭示了其在发育过程中调控细胞存亡的双重功能。
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以上文献均通过UNC5B抗体在特定实验模型中探索其生物学功能，涵盖血管生成、肿瘤抑制、神经发育及凋亡调控等领域。
UNC5B antibody is a research tool targeting the UNC5B receptor, a key protein in the netrin signaling pathway. UNC5B, also known as UNC5H2. belongs to the UNC5 family of dependence receptors that regulate cellular processes such as axon guidance, cell migration, and apoptosis. Structurally, it contains extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic death domain. UNC5B typically binds netrin-1 to promote cell survival and guidance, but in the absence of netrin, it triggers caspase-dependent apoptosis.
This receptor plays critical roles in vascular and neural development, with studies linking it to angiogenesis, blood vessel patterning, and nervous system wiring. In cancer research, UNC5B exhibits dual roles: it may act as a tumor suppressor by inducing apoptosis in netrin-deficient microenvironments, while also potentially promoting metastasis in certain contexts. Dysregulation of UNC5B has been associated with cardiovascular diseases, neurodevelopmental disorders, and tumor progression.
UNC5B antibodies are widely used in techniques like Western blot, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and interaction partners. They enable investigations into UNC5B's tissue distribution, signaling mechanisms, and therapeutic potential. Recent studies also explore its involvement in atherosclerosis and metabolic diseases, highlighting its broad biological relevance. Validation of antibody specificity remains crucial due to structural similarities within the UNC5 receptor family.
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