| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor RFX4, Regulatory factor X 4, Testis development protein NYD-SP10, RFX4 |
| Entrez GeneID | 5992 |
| WB Predicted band size | 83.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RFX4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 684-720 amino acids from the C-terminal region of human RFX4. |
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以下是3篇关于RFX4抗体的参考文献及其摘要概括：
1. **文献名称**: *RFX4 regulates ciliogenesis and brain development in mice*
**作者**: Baas D et al.
**摘要**: 本研究通过RFX4抗体免疫染色发现，RFX4转录因子在小鼠胚胎神经管纤毛形成中起关键作用，其缺失导致脑室结构异常和胚胎致死，证实了RFX4对中枢神经系统发育的调控功能。
2. **文献名称**: *A novel role for RFX4 in medulloblastoma tumorigenesis*
**作者**: Lee JC et al.
**摘要**: 利用RFX4抗体进行Western blot和免疫组化分析，发现RFX4在髓母细胞瘤中表达异常，其下调通过激活Sonic Hedgehog信号通路促进肿瘤生长，提示RFX4可能作为抑癌基因发挥作用。
3. **文献名称**: *Genome-wide analysis of RFX4 binding sites in choroid plexus epithelium*
**作者**: Wang Y et al.
**摘要**: 通过RFX4抗体的染色质免疫沉淀测序(ChIP-seq)，揭示了RFX4在脉络丛上皮细胞中调控脑脊液分泌相关基因的分子机制，为脑-脑脊液屏障研究提供新方向。
4. **文献名称**: *RFX4 variants cause X-linked hydrocephalus via disrupting ciliary gene regulation*
**作者**: Smith RS et al.
**摘要**: 研究利用患者来源细胞系中的RFX4抗体染色，发现X染色体连锁的RFX4突变导致纤毛相关基因表达异常，从而引发先天性脑积水，为遗传性神经发育障碍提供病理机制解释。
注：上述文献信息为基于RFX4相关研究的概括性模拟，实际文献需通过PubMed等数据库检索确认。
The RFX4 antibody is a research tool designed to detect and study the RFX4 protein, a member of the regulatory factor X (RFX) family of transcription factors. RFX proteins are characterized by a conserved DNA-binding domain that recognizes X-box motifs in gene promoters, regulating diverse biological processes. RFX4. specifically, plays critical roles in embryonic development, particularly in the formation of the central nervous system (CNS) and notochord. Studies suggest its involvement in ciliogenesis, brain morphogenesis, and the maintenance of the ventricular system. Dysregulation of RFX4 has been linked to developmental disorders and cancers, including gliomas and medulloblastoma.
The RFX4 antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize RFX4 expression patterns in tissues or cultured cells. Its applications span developmental biology, neuroscience, and oncology, aiding in the exploration of RFX4's molecular mechanisms and interactions. Commercial RFX4 antibodies are typically raised against specific epitopes, such as peptide sequences within its N-terminal or C-terminal regions, and validated for species reactivity (e.g., human, mouse). Researchers rely on this tool to elucidate RFX4's role in health and disease, contributing to potential therapeutic insights for related pathologies.
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