| WB | 1/2000-1/8000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rabenosyn-5, 110 kDa protein, FYVE finger-containing Rab5 effector protein rabenosyn-5, RAB effector RBSN {ECO:0000312|HGNC:HGNC:20759}, Zinc finger FYVE domain-containing protein 20, RBSN (HGNC:20759) |
| Entrez GeneID | 64145 |
| WB Predicted band size | 88.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ZFYVE20 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 41-65 amino acids from the human region of human ZFYVE20. |
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以下是关于ZFYVE20 (N-Term)抗体的3篇文献示例(注：文献为示例性描述，可能非真实存在)：
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1. **文献名称**：*"ZFYVE20 regulates endosomal trafficking through its FYVE domain: Antibody validation and functional analysis"*
**作者**：Smith A, et al.
**摘要**：本研究利用针对ZFYVE20 N端的多克隆抗体，验证了其在HeLa细胞中的内体定位。通过免疫荧光和Western blot分析，证实ZFYVE20通过FYVE结构域与磷脂酰肌醇-3-磷酸结合，调控内体-溶酶体运输通路。
2. **文献名称**：*"Development of a monoclonal antibody targeting ZFYVE20 N-terminal region for hepatocellular carcinoma diagnosis"*
**作者**：Chen L, et al.
**摘要**：研究团队开发了一种靶向ZFYVE20 N端的高特异性单克隆抗体，并应用于肝细胞癌(HCC)组织样本的免疫组化检测。结果显示ZFYVE20在HCC中高表达，且与患者预后不良相关。
3. **文献名称**：*"ZFYVE20 interacts with autophagy machinery: Insights from co-immunoprecipitation studies using N-terminal specific antibodies"*
**作者**：Yamamoto K, et al.
**摘要**：通过使用ZFYVE20 N端抗体进行免疫共沉淀实验，发现ZFYVE20与自噬相关蛋白LC3和ATG5存在相互作用，提示其在细胞自噬调控中的潜在作用。
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以上文献示例概括了ZFYVE20抗体在定位验证、疾病诊断及分子机制研究中的应用。实际文献需通过学术数据库(如PubMed、Google Scholar)检索确认。
The ZFYVE20 (N-Term) antibody is designed to target the N-terminal region of the ZFYVE20 protein, a member of the FYVE domain-containing protein family. ZFYVE20. also known as protrudin, plays a critical role in intracellular membrane trafficking, neurite outgrowth, and endoplasmic reticulum (ER) dynamics. The FYVE domain, a zinc-binding motif, enables interaction with phosphatidylinositol 3-phosphate (PI3P), anchoring the protein to endosomal membranes and facilitating vesicle transport and autophagy. The N-terminal region of ZFYVE20 is involved in binding to molecular partners like Rab11 and Sec61β, influencing ER-to-Golgi transport and neuronal development.
This antibody is commonly used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study ZFYVE20 expression, localization, and function in cellular models or tissues. It has been particularly valuable in neuroscience and cancer research, where dysregulation of ZFYVE20 is linked to neurodegenerative disorders, tumor metastasis, and impaired autophagy. Researchers also utilize it to explore ZFYVE20’s role in lipid metabolism and its interaction with signaling pathways, including mTOR and PI3K/AKT.
Validated for specificity and sensitivity, the ZFYVE20 (N-Term) antibody aids in elucidating the protein’s mechanistic contributions to cellular homeostasis and disease pathology.
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