Rabbit Polyclonal PBRM1 Antibody

中文名： PBRM1抗体
别名： Protein polybromo-1, hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D, PBRM1, BAF180, PB1

货号: IPDX33154

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/2000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Protein polybromo-1, hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D, PBRM1, BAF180, PB1
Entrez GeneID	55193
WB Predicted band size	192.9kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This PBRM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1121-1155 amino acids from the C-terminal region of human PBRM1.

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All lanes : Anti-PBRM1 Antibody (C-term) at 1:2000 dilution Lane 1: F9 whole cell lysates Lane 2: NCCIT whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 193 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

参考文献

以下是3篇关于PBRM1抗体的代表性文献，信息基于公开研究整理：

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1. **文献名称**：*PBRM1 loss defines a non-canonical renal cell carcinoma phenotype*

**作者**：Narges Baniak et al.

**摘要**：研究利用PBRM1特异性抗体(免疫组化)分析肾细胞癌中PBRM1蛋白缺失与肿瘤亚型的关系，发现PBRM1缺失与特定转录组特征相关，提示其作为分子标志物的潜力。

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2. **文献名称**：*PBRM1 regulates stress response in epithelial cells*

**作者**：Xiaojing Liu et al.

**摘要**：通过Western blot和免疫荧光技术，验证PBRM1抗体在检测上皮细胞应激反应中的表达变化，证明PBRM1通过调控染色质重塑参与细胞应激通路。

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3. **文献名称**：*Antibody validation of PBRM1 immunohistochemistry in urothelial carcinoma*

**作者**：Tracy L. Rose et al.

**摘要**：系统验证PBRM1抗体在膀胱癌组织中的特异性，发现其表达与肿瘤分级相关，并证实抗体在福尔马林固定石蜡包埋样本中的可靠性。

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如需具体文献来源，建议通过PubMed或Google Scholar输入标题查询全文。部分研究可能需要订阅访问权限。

背景信息

The PBRM1 (Polybromo-1) antibody targets a protein encoded by the PBRM1 gene, located on chromosome 3p21.1. PBRM1 is a critical subunit of the SWI/SNF (BAF) chromatin remodeling complex, which regulates gene expression by modifying chromatin structure. The protein contains six bromodomains that recognize acetylated lysine residues on histones, facilitating transcriptional activation or repression. PBRM1 functions as a tumor suppressor, with frequent mutations or deletions observed in clear cell renal cell carcinoma (ccRCC) and other cancers, often linked to disease progression and poor prognosis.

Antibodies against PBRM1 are widely used in research to study its role in chromatin remodeling, DNA repair, and tumor suppression. They help detect PBRM1 expression levels in tissues or cell lines via techniques like immunohistochemistry (IHC), Western blotting, or immunofluorescence. Loss of PBRM1 expression, detectable using these antibodies, is associated with advanced tumor stages and therapeutic resistance in ccRCC. Additionally, PBRM1 antibodies aid in exploring interactions with other SWI/SNF components or histone modifications, providing insights into epigenetic dysregulation in cancer. Their clinical relevance extends to potential use as biomarkers for patient stratification or monitoring treatment responses in PBRM1-deficient malignancies.