Rabbit Polyclonal ITGA7(N-term) Antibody

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     All lanes : Anti-ITGA7 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: A431 whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 129 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling ITGA7 with P33153 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG  secondary antibody at 1/200 dilution (green). Immunofluorescence image showing vesicles,cytoplasm and weakly nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing U-2 OS cells stained with P33153 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33153,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P33153 staining ITGA7 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The ITGA7 (N-term) antibody targets the N-terminal region of integrin alpha-7 (ITGA7), a key subunit of the α7β1 integrin heterodimer. Integrins are transmembrane receptors mediating cell-extracellular matrix (ECM) adhesion and signaling. ITGA7 is predominantly expressed in skeletal and cardiac muscle, where it binds laminin in the basement membrane, playing a critical role in muscle cell adhesion, differentiation, and maintenance. The α7β1 integrin stabilizes the muscle membrane during contraction and supports myogenic stem cell function, making it essential for muscle development and repair.

ITGA7 (N-term) antibodies are widely used in research to study ITGA7 expression, localization, and function via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help investigate ITGA7's involvement in muscular dystrophies, cardiomyopathies, and muscle regeneration processes. Dysregulation of ITGA7 is linked to diseases such as congenital myopathy and fibrosis, highlighting its potential as a therapeutic target or biomarker.

Specificity of ITGA7 (N-term) antibodies is typically validated using knockout controls or peptide blocking assays. Cross-reactivity varies by product; many are validated for human, mouse, and rat samples. Researchers should verify species compatibility and application-specific performance. Overall, ITGA7 (N-term) antibodies serve as vital tools for exploring integrin-mediated ECM interactions in muscle physiology and pathology.