| WB | 1/2000 | Mouse, Rat |
| IF | 咨询技术 | Mouse, Rat |
| IHC | 咨询技术 | Mouse, Rat |
| ICC | 技术咨询 | Mouse, Rat |
| FCM | 咨询技术 | Mouse, Rat |
| Elisa | 咨询技术 | Mouse, Rat |
| Aliases | P2Y purinoceptor 4, P2Y4, P2ry4, P2y4r |
| Entrez GeneID | 57385 |
| WB Predicted band size | 41.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse, Rat |
| Immunogen | This P2ry4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-42 amino acids from the N-terminal region of human P2ry4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于P2RY4(N-term)抗体的模拟参考文献示例(注：以下内容为假设性示例，实际文献可能需要进一步检索验证)：
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1. **文献名称**: *Development of a Specific Polyclonal Antibody Against the N-Terminal Domain of P2Y4 Receptor*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对人P2RY4受体N端表位的兔多克隆抗体。通过免疫印迹和免疫荧光验证其特异性，证实其在HEK293过表达细胞系及结肠组织中可特异性识别P2RY4.为受体定位研究提供了工具。
2. **文献名称**: *Localization of P2Y4 Receptor in Intestinal Epithelium Using N-Terminal Antibody*
**作者**: Jones B, et al.
**摘要**: 利用抗P2RY4 N端抗体，揭示了该受体在小肠上皮细胞顶膜的高表达，提示其可能参与ATP介导的离子转运调控，并探讨了其在肠道炎症中的潜在作用。
3. **文献名称**: *P2Y4 Receptor Signaling in Cancer: Insights from Antibody-Based Detection*
**作者**: Lee C, et al.
**摘要**: 通过抗P2RY4 N端抗体发现，该受体在乳腺癌细胞膜和胞内囊泡中表达，并证明其激活可促进ERK磷酸化，为靶向P2RY4的肿瘤治疗策略提供依据。
4. **文献名称**: *Comparative Validation of P2Y4 Antibodies Highlights Challenges in Specificity*
**作者**: Davis D, et al.
**摘要**: 系统评估了多种市售P2RY4抗体(包括N端靶向抗体)的特异性，发现部分抗体存在交叉反应，建议结合基因敲除模型验证结果，强调抗体选择对研究可靠性的影响。
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**注意**：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献，并依据实验需求核实抗体信息。
The P2RY4 (N-term) antibody is designed to target the N-terminal region of the P2Y4 receptor, a member of the purinergic receptor family. P2Y receptors are G protein-coupled receptors (GPCRs) that respond to extracellular nucleotides like ATP, UTP, and their derivatives, playing critical roles in cellular signaling pathways. P2Y4. specifically, is activated by uridine triphosphate (UTP) and couples to Gq proteins, triggering phospholipase C (PLC) activation, intracellular calcium mobilization, and protein kinase C (PKC) signaling. It is expressed in various tissues, including the gastrointestinal tract, immune cells, and the central nervous system, where it regulates processes such as ion transport, inflammation, and neurotransmission.
The N-terminal domain of P2Y4 is extracellular and contributes to ligand binding and receptor activation. Antibodies targeting this region are valuable tools for studying receptor localization, expression levels, and functional interactions in physiological and pathological contexts. For example, they are used in techniques like Western blotting, immunohistochemistry, and flow cytometry to investigate P2Y4's role in diseases such as cancer, inflammatory disorders, or metabolic syndromes. By enabling precise detection, the P2RY4 (N-term) antibody aids in elucidating the receptor's regulatory mechanisms and potential therapeutic applications. Its specificity for the N-terminus ensures minimal cross-reactivity with other P2Y subtypes, enhancing experimental accuracy.
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