| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Ezh2, Enx1h |
| Entrez GeneID | 14056 |
| WB Predicted band size | 85.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Ezh2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 260-295 amino acids from the Central region of Mouse Ezh2. |
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以下是3-4条关于小鼠Ezh2抗体的参考文献及其摘要概述：
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1. **文献名称**: "Ezh2 Requires PHF1 To Efficiently Catalyze H3K27 Trimethylation In Vivo"
**作者**: Margueron, R. & Reinberg, D.
**摘要**: 研究揭示了Ezh2(组蛋白甲基转移酶)与PHF1蛋白的相互作用对其催化H3K27三甲基化(H3K27me3)的功能至关重要，并通过小鼠模型和小鼠来源的Ezh2抗体验证了其在染色质修饰复合体PRC2中的核心作用。
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2. **文献名称**: "Targeting EZH2 in Cancer"
**作者**: Kim, K.H. & Roberts, C.W.
**摘要**: 该文献利用小鼠Ezh2抗体进行免疫沉淀和Western blot分析，证明Ezh2在多种癌症中异常高表达，并通过基因敲除实验表明抑制Ezh2可减缓肿瘤生长，为癌症治疗提供了潜在靶点。
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3. **文献名称**: "Antibody-based detection of Ezh2 phosphorylation states in mouse embryonic stem cells"
**作者**: Su, I.H. et al.
**摘要**: 开发了一种特异性识别小鼠Ezh2磷酸化状态的抗体，结合流式细胞术和免疫荧光技术，揭示了Ezh2在不同细胞分化阶段的动态修饰及其对多能性维持的调控。
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4. **文献名称**: "EZH2 is essential for the generation of germinal center B cells"
**作者**: Béguelin, W. et al.
**摘要**: 通过小鼠模型和Ezh2特异性抗体染色，证明Ezh2通过调控H3K27me3修饰参与生发中心B细胞的分化，缺失Ezh2会导致免疫应答缺陷，突显其在体液免疫中的关键作用。
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以上文献均聚焦于Ezh2的功能、抗体应用及其在表观遗传调控和疾病中的机制。
The enhancer of zeste homolog 2 (Ezh2) is a critical histone methyltransferase and a core component of the Polycomb Repressive Complex 2 (PRC2), which mediates gene silencing by catalyzing the trimethylation of histone H3 at lysine 27 (H3K27me3). This epigenetic modification plays a pivotal role in regulating chromatin structure, stem cell maintenance, differentiation, and cellular identity. Dysregulation of Ezh2 is implicated in various cancers, developmental disorders, and other diseases, making it a key target for biomedical research.
Mouse Ezh2 antibodies are widely used tools to study the expression, localization, and functional roles of Ezh2 in murine models. These antibodies enable the detection of Ezh2 protein via techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Given the high homology between mouse and human Ezh2. findings from murine studies often provide insights into human biology and disease mechanisms.
Researchers employ Ezh2 antibodies to investigate its involvement in processes like embryonic development, tumor progression, and epigenetic reprogramming. Specificity and validation are crucial, as cross-reactivity with related proteins (e.g., Ezh1) can confound results. High-quality Ezh2 antibodies are essential for advancing studies in cancer therapeutics, stem cell biology, and regenerative medicine, where targeting Ezh2-mediated epigenetic pathways holds therapeutic promise.
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