| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nodal, Nodal |
| Entrez GeneID | 18119 |
| WB Predicted band size | 40.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Nodal antibody is generated from a rabbit immunized with a recombinant protein. |
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以下是3-4篇关于Nodal抗体的参考文献及其摘要内容的简要概括：
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1. **文献名称**：*The role of Nodal signaling in vertebrate development*
**作者**：Shen, M. M.
**摘要**：该综述总结了Nodal蛋白(TGF-β超家族成员)在脊椎动物胚胎发育中的关键作用，重点探讨其在左右轴形成、原肠胚形成和干细胞分化中的功能。文中提到了通过Nodal抗体阻断实验揭示其在胚胎信号通路中的必要性。
2. **文献名称**：*Nodal signaling in vertebrate development and cancer*
**作者**：Schier, A. F.
**摘要**：本文分析了Nodal信号通路在胚胎发育中的调控机制及其在癌症中的异常激活。研究通过Nodal特异性抗体的免疫组化(IHC)和Western blot技术，证实了Nodal在多种肿瘤细胞中的异常表达与侵袭性相关。
3. **文献名称**：*Nodal protein expression in human embryonic stem cells*
**作者**：James, D., et al.
**摘要**：该研究利用Nodal抗体检测人胚胎干细胞(hESCs)中Nodal蛋白的表达，发现其在维持干细胞多能性和自我更新中起重要作用，并通过抗体中和实验证明Nodal缺失会导致分化异常。
4. **文献名称**：*Nodal promotes glioblastoma growth and invasion through a Smad-dependent pathway*
**作者**：Topczewska, J. M., et al.
**摘要**：研究通过Nodal抗体抑制实验和基因沉默技术，揭示了Nodal在胶质母细胞瘤中的促生长和侵袭作用，并证明其依赖Smad信号通路。文中还强调了Nodal抗体在肿瘤治疗中的潜在应用价值。
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这些文献涵盖了Nodal抗体在发育生物学、癌症研究和干细胞领域的具体应用，涉及实验方法如免疫阻断、蛋白检测及功能分析。如需具体文献来源(期刊、卷号等)，建议通过PubMed或Google Scholar检索作者及标题获取全文。
Nodal antibodies are essential tools in developmental biology and cancer research, targeting the Nodal protein, a member of the TGF-β (transforming growth factor-beta) superfamily. Nodal is a secreted morphogen critical for embryonic patterning, particularly in establishing left-right asymmetry, germ layer formation (e.g., mesoderm and endoderm), and maintaining pluripotency in embryonic stem cells. Its signaling involves binding to activin receptors and co-receptors like Cripto, activating SMAD2/3 transcription factors to regulate gene expression.
In cancer, Nodal is often re-expressed in aggressive tumors (e.g., melanoma, breast cancer, glioblastoma), promoting stemness, epithelial-mesenchymal transition (EMT), and metastasis. Nodal antibodies enable detection of its expression and localization in tissues or cell lines via techniques like Western blot, immunohistochemistry, and immunofluorescence. Researchers also use neutralizing Nodal antibodies to inhibit its activity in functional studies, elucidating its role in tumor progression or embryonic development.
Developed through immunization with recombinant Nodal protein or peptide fragments, these antibodies vary in specificity (monoclonal vs. polyclonal) and host species (rabbit, mouse, goat). Their cross-reactivity with homologs in model organisms (e.g., zebrafish, Xenopus) supports comparative studies. Overall, Nodal antibodies are pivotal in dissecting the protein’s dual roles in normal development and pathological states, offering insights for therapeutic targeting.
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