| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable E3 ubiquitin-protein ligase HERC4, 632-, HECT domain and RCC1-like domain-containing protein 4, HERC4, KIAA1593 |
| Entrez GeneID | 26091 |
| WB Predicted band size | 118.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This HERC4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 445-479 amino acids from the Central region of human HERC4. |
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以下是关于HERC4抗体的3篇参考文献示例(注：以下内容为模拟生成，非真实存在的文献)：
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1. **文献名称**：*HERC4 ubiquitin ligase function in DNA repair and cancer progression*
**作者**：Schulman BA, et al.
**摘要**：本研究解析了HERC4作为E3泛素连接酶的结构特征，发现其通过调控BRCA1蛋白的稳定性参与DNA损伤修复。利用特异性抗体敲低HERC4后，癌细胞对放疗敏感性显著增加。
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2. **文献名称**：*Development of a monoclonal antibody targeting HERC4 for virology studies*
**作者**：McLaughlin-Drubin ME, et al.
**摘要**：文章报道了一种高特异性的兔源抗HERC4单克隆抗体的开发，并验证其在免疫共沉淀(Co-IP)中的应用。通过该抗体发现HERC4与HPV E7蛋白存在相互作用，提示其在病毒感染中的潜在作用机制。
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3. **文献名称**：*HERC4 overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Sasaki T, et al.
**摘要**：利用免疫组化(IHC)结合抗HERC4抗体，发现肝癌组织中HERC4表达水平与患者生存率呈负相关。体外实验表明，HERC4抗体介导的蛋白降解可抑制肝癌细胞迁移。
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(注：如需真实文献，建议通过PubMed或Google Scholar检索关键词“HERC4 antibody”、“HERC4 ubiquitin ligase”等。)
The HERC4 antibody is a tool used to study the HERC4 protein, a member of the HERC (Homologous to the E6-AP Carboxyl Terminus and RCC1-like Domain) family. HERC proteins are characterized by their conserved HECT (homologous to E6-AP C-terminus) domain, which confers E3 ubiquitin ligase activity, and RCC1-like domains (RLDs) involved in guanine nucleotide exchange. HERC4. also known as UBE3C, is less well-characterized compared to other HERC family members like HERC1 or HERC2. It is implicated in ubiquitination pathways, potentially regulating protein degradation, cellular signaling, and immune responses. Studies suggest roles in antiviral defense, cell cycle progression, and spermatogenesis.
The HERC4 antibody is essential for detecting and quantifying HERC4 expression in research settings. It is commonly used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate protein localization, interaction partners, and functional mechanisms. Commercial HERC4 antibodies are typically raised in rabbits or mice against specific epitopes of the human HERC4 protein, with cross-reactivity tested in model organisms like mice or rats. Validation often includes knockout/knockdown controls or overexpression assays to confirm specificity.
Despite its utility, research on HERC4 remains limited, and its precise biological roles and disease associations (e.g., cancer, neurodegeneration) require further exploration. The antibody serves as a critical reagent for unraveling HERC4's contributions to cellular homeostasis and pathology.
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