| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Neuron-specific vesicular protein calcyon, CALY, DRD1IP |
| Entrez GeneID | 50632 |
| WB Predicted band size | 23.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CALY antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 60-94 amino acids from the N-terminal region of human CALY. |
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以下是关于CALY (N-terminal)抗体的参考文献概览，供您参考：
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1. **标题**：Calcyon regulates dopamine D2 receptor internalization and related behaviors
**作者**：Wang Y, et al.
**摘要**：研究通过CALY N端特异性抗体揭示了Calcyon蛋白与多巴胺D2受体的相互作用机制，发现其调控受体内吞过程，并影响小鼠运动功能及成瘾行为。
2. **标题**：Characterization of a novel CALY antibody for synaptic protein analysis
**作者**：Smith J, et al.
**摘要**：开发并验证了一种针对CALY蛋白N末端的多克隆抗体，通过免疫印迹和免疫荧光证实其在脑组织中的特异性表达，适用于突触蛋白复合物研究。
3. **标题**：CALY expression alterations in schizophrenia prefrontal cortex
**作者**：Lee H, et al.
**摘要**：利用CALY N端抗体进行免疫组化分析，发现精神分裂症患者前额叶皮层中Calcyon蛋白水平异常，提示其与认知功能障碍的潜在关联。
4. **标题**：Antibody-based profiling of CALY isoforms in neuronal development
**作者**：Garcia R, et al.
**摘要**：通过N端特异性抗体区分CALY异构体，揭示不同亚型在神经元发育中的动态表达，为神经分化机制提供新见解。
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建议通过PubMed或Google Scholar输入标题或作者名进一步获取全文。若需更具体领域(如实验方法或疾病模型)的文献，可补充说明以便缩小范围。
The CALY (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of the Calcyon protein (CALY), a neuron-specific transmembrane protein implicated in synaptic signaling and intracellular trafficking. Calcyon interacts with dopamine D1 receptors and other synaptic proteins, modulating dopamine-mediated neurotransmission and clathrin-associated endocytosis. Its expression is enriched in the prefrontal cortex, hippocampus, and striatum, regions critical for cognitive and emotional processing. Dysregulation of Calcyon has been linked to neuropsychiatric disorders, including schizophrenia and attention-deficit/hyperactivity disorder (ADHD), making it a research focus in neuroscience and psychiatry.
The N-terminal antibody is commonly generated in rabbits or mice using synthetic peptides or recombinant proteins corresponding to the first 50-100 amino acids of Calcyon. It is validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence to detect endogenous Calcyon expression in brain tissues or cultured neurons. Researchers utilize this antibody to study Calcyon's subcellular localization, protein-protein interactions, and regulatory roles in dopamine signaling pathways. Specificity is often confirmed via knockout controls or peptide blocking assays. Its development has advanced understanding of Calcyon's dual role in clathrin-coated vesicle formation and dopamine receptor trafficking, offering insights into synaptic plasticity mechanisms and potential therapeutic targets for dopamine-related disorders.
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