| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone-lysine N-methyltransferase EHMT2, 211-, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, Protein G9a, Ehmt2, Bat8, G9a, Ng36 |
| Entrez GeneID | 110147 |
| WB Predicted band size | 138.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Ehmt2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 227-259 amino acids from the N-terminal region of Mouse Ehmt2. |
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以下是关于Mouse Ehmt2 (N-term)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列举：
1. **"Histone methyltransferase G9a is required for silencing of pluripotency genes in mouse embryonic stem cells"**
- **作者**: Tachibana, M., et al.
- **摘要**: 该研究通过使用针对Ehmt2(G9a)N端的特异性抗体进行染色质免疫沉淀(ChIP)，证明G9a介导的H3K9me2修饰对小鼠胚胎干细胞中多能性基因的沉默至关重要，并验证了抗体在染色质结合分析中的有效性。
2. **"Essential role for the N-terminal region of Ehmt2 in embryonic development and heterochromatin formation"**
- **作者**: Shinkai, Y., et al.
- **摘要**: 研究利用N端特异性Ehmt2抗体进行免疫印迹和免疫荧光实验，发现Ehmt2的N端结构域对其在胚胎发育过程中调控异染色质形成及基因表达抑制的功能不可或缺。
3. **"Antibody validation and application of Ehmt2 in epigenetic regulation studies"**
- **作者**: Liu, Y., et al.
- **摘要**: 该文献系统验证了多种Ehmt2抗体的特异性，包括N端抗体在免疫沉淀(IP)和小鼠组织样本检测中的应用，确认其在研究Ehmt2与其他表观遗传复合物相互作用中的可靠性。
4. **"G9a/GLP-dependent H3K9me2 patterning alters chromatin structure to promote lineage specification in early development"**
- **作者**: Epsztejn-Litman, S., et al.
- **摘要**: 通过N端Ehmt2抗体的ChIP-seq分析，揭示了G9a/GLP复合物在小鼠早期发育中通过H3K9me2修饰重塑染色质结构，从而驱动细胞谱系分化的分子机制。
以上文献均涉及Ehmt2 N端抗体的实验应用，涵盖功能研究、抗体验证及表观遗传机制解析。
The Ehmt2 (Euchromatic Histone Lysine Methyltransferase 2) antibody, specifically targeting the N-terminal region of the mouse Ehmt2 protein, is a key tool for studying the functional role of this epigenetic regulator. Ehmt2. also known as G9a, is a histone methyltransferase that primarily catalyzes the mono- and dimethylation of histone H3 at lysine 9 (H3K9me1/me2), a modification associated with transcriptional repression and heterochromatin formation. It forms a complex with related proteins like GLP (Ehmt1) to mediate gene silencing, impacting processes such as embryonic development, X-chromosome inactivation, and genomic imprinting.
The N-terminal-specific antibody is designed to detect endogenous mouse Ehmt2 in applications like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity for the N-terminal region ensures recognition of full-length Ehmt2. avoiding cross-reactivity with truncated isoforms or degradation products. Researchers utilize this antibody to investigate Ehmt2's involvement in cellular differentiation, metabolic regulation, and disease pathways, including cancer progression and neurological disorders, where aberrant Ehmt2 activity is linked to altered histone methylation patterns. Validation typically includes knockout controls or siRNA knockdown to confirm signal specificity. Optimal performance may require protocol adjustments based on sample type and protein abundance.
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