| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rhox homeobox family member 1, Ovary-, testis- and epididymis-expressed gene protein, Paired-like homeobox protein PEPP-1, RHOXF1, OTEX, PEPP1 |
| Entrez GeneID | 158800 |
| WB Predicted band size | 20.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RHOXF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 86-120 amino acids from the Central region of human RHOXF1. |
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以下是3篇与RHOXF1抗体相关的模拟参考文献(基于真实研究领域方向，但文献标题及作者为模拟简化)：
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1. **文献名称**: *RHOXF1 as a novel biomarker in germ cell tumors: Immunohistochemical analysis using a monoclonal antibody*
**作者**: Müller J, et al.
**摘要**: 研究利用自主研发的小鼠抗人RHOXF1单克隆抗体，通过免疫组化分析发现RHOXF1在精原细胞瘤和胚胎癌中高表达，提示其可作为生殖细胞肿瘤的新型诊断标志物。
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2. **文献名称**: *RHOXF1 antibody validation in transcriptional regulation of human embryonic stem cells*
**作者**: Chen L, et al.
**摘要**: 通过ChIP-seq和Western blot验证兔多克隆RHOXF1抗体的特异性，证明RHOXF1结合人类胚胎干细胞中OCT4和NANOG基因启动子区，调控多能性相关通路。
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3. **文献名称**: *Comparative study of RHOXF1 and RHOXF2/2B antibody specificity in primate tissues*
**作者**: Tanaka K, et al.
**摘要**: 研究对比了多种商业RHOXF1抗体在人类及非人灵长类组织中的交叉反应性，发现部分抗体与RHOXF2/2B存在交叉识别，强调了抗体验证在生殖发育研究中的必要性。
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注：以上文献为模拟简化版本，实际研究中建议通过PubMed或Google Scholar以关键词“RHOXF1 antibody”、“RHOXF1 biomarker”检索真实文献。
The RHOXF1 antibody is a tool used to detect the presence of RHOXF1 (Rhox homeobox family member 1), a protein encoded by the RHOXF1 gene. This gene belongs to the Rhox family of homeobox genes, which are clustered on the X chromosome and primarily expressed in reproductive tissues. RHOXF1. also known as PEPP2. is specifically associated with germ cell development and is predominantly expressed in the testis and placenta. It plays roles in gametogenesis, embryogenesis, and potentially in regulating stem cell pluripotency.
RHOXF1 antibodies are typically polyclonal or monoclonal, generated using immunogenic peptides or recombinant protein fragments. These antibodies are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study RHOXF1's expression patterns, localization, and function in cellular models or tissues. Specificity is confirmed using knockout controls or siRNA-mediated knockdown.
Research involving RHOXF1 antibodies has linked this protein to reproductive disorders and cancers, including testicular and ovarian cancers, where its dysregulation may contribute to tumorigenesis. Its restricted expression makes it a potential biomarker for germ cell-related pathologies. However, commercial availability of high-quality RHOXF1 antibodies remains limited, requiring thorough validation for experimental reliability.
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