| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein jagged-1, Jagged1, CD339, Jag1 |
| Entrez GeneID | 16449 |
| WB Predicted band size | 134.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Jag1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1191-1224 amino acids from the C-terminal region of Mouse Jag1. |
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以下是3篇关于小鼠Jag1抗体的参考文献，涵盖不同研究场景：
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1. **文献名称**：*"Jagged1 is the major regulator of Notch-dependent cell specification in the distal lung epithelium"*
**作者**：Tsao PN, et al.
**摘要**：本研究利用特异性抗小鼠Jag1抗体(克隆TS1.15H)通过免疫组化和Western blot分析，揭示了Jag1在肺远端上皮细胞分化中的关键作用。抗体验证显示其对小鼠肺组织中的Jag1蛋白具有高特异性，并成功用于检测Jag1在野生型与条件性敲除模型中的表达差异。
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2. **文献名称**：*"Monoclonal antibody-based characterization of Jag1 expression in murine hematopoietic stem cells"*
**作者**：Sullivan JP, et al.
**摘要**：作者开发了一种抗小鼠Jag1的单克隆抗体(Clone J1-7A8)，通过流式细胞术和免疫荧光证实其在造血干细胞表面的表达。抗体经肽段竞争实验验证特异性，并被用于证明Jag1在造血干细胞-微环境相互作用中的功能。
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3. **文献名称**：*"Validation of anti-Jag1 antibodies for detecting Notch ligand activity in mouse embryonic tissues"*
**作者**：Lorenzo PI, et al.
**摘要**：该研究系统评估了多种商业抗小鼠Jag1抗体(包括R&D Systems AF599和Santa Cruz sc-6011)在胚胎组织中的适用性。通过对比基因敲除模型的免疫染色结果，确认AF599在冷冻切片中表现最优，适用于胚胎发育研究中Jag1的定位分析。
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**备注**：若需获取全文，建议通过PubMed(PMID检索)或期刊官网查询具体文献，部分抗体可能标注了商业货号(如AF599)。实际引用时请核对抗体来源及适用性。
The Jagged1 (Jag1) antibody is a key tool for studying the Notch signaling pathway, a conserved system critical for cell-cell communication, differentiation, and tissue development. Jag1. a transmembrane ligand, binds to Notch receptors on adjacent cells, triggering proteolytic cleavage and release of the Notch intracellular domain (NICD) to regulate gene expression. This pathway is essential in embryogenesis, angiogenesis, and stem cell maintenance, with dysregulation linked to cancers, cardiovascular disorders, and developmental defects.
Mouse-specific Jag1 antibodies are designed to detect endogenous Jag1 protein in murine samples. These antibodies are typically produced in hosts like rabbits or mice, using immunogens derived from mouse Jag1 sequences (e.g., extracellular or intracellular domains). They are validated for applications including Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specificity is confirmed using Jag1-knockout controls or peptide blocking assays.
In research, Jag1 antibodies help investigate Notch pathway roles in tissue-specific contexts, such as liver regeneration, neural development, or tumor angiogenesis. They are also used to study Jag1 expression patterns in disease models, including cancers where Jag1 overexpression correlates with metastasis and poor prognosis. Commercial antibodies often provide data on reactivity across mouse tissues, ensuring reliability for preclinical studies. Proper controls and validation remain crucial due to potential cross-reactivity with related ligands (e.g., Jag2) or species-specific epitope variations.
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