Rabbit Polyclonal MouseAlk(P1517) Antibody

The Mouse Alk (P1517) antibody is a monoclonal antibody specifically designed to detect anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase belonging to the insulin receptor superfamily. ALK plays critical roles in neural development and synaptic plasticity. Dysregulated ALK signaling, caused by gene fusions (e.g., *EML4-ALK*), mutations, or overexpression, is implicated in oncogenesis, including anaplastic large cell lymphoma (ALCL), neuroblastoma, and non-small cell lung cancer (NSCLC). The P1517 clone recognizes an epitope within the intracellular domain of ALK, enabling the detection of both wild-type and fusion variants in mouse-derived samples. It is widely used in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study ALK expression, activation, and subcellular localization in disease models. The antibody’s specificity is validated using ALK-positive cell lines and tissues, with knockout controls confirming minimal cross-reactivity. Storage typically requires aliquoting at -20°C to preserve activity. Researchers employ this tool to explore ALK’s role in tumor progression, evaluate targeted therapies (e.g., ALK inhibitors like crizotinib), and validate preclinical models. Its reliability in murine systems makes it valuable for translational studies linking ALK dysregulation to therapeutic resistance or metastasis mechanisms.