| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 20111 |
| WB Predicted band size | 81.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This mouse Rps6ka1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 704-738 amino acids from the C-terminal region of mouse Rps6ka1. |
+ +
1. **"Characterization of Rps6ka1 (RSK1) knockout mice and its role in neuronal differentiation"**
*Author: Smith J, et al. (2018)*
*摘要：* 该研究利用特异性Rps6ka1抗体，通过Western blot和免疫组化分析，揭示了RSK1在小鼠海马神经元分化中的关键作用，并验证了抗体在小鼠脑组织中的特异性。
2. **"RSK1-mediated signaling in tumor progression: Insights from mouse xenograft models"**
*Author: Chen L, et al. (2020)*
*摘要：* 通过小鼠异种移植模型，使用抗Rps6ka1抗体检测肿瘤组织中RSK1的磷酸化状态，证明其通过调控MAPK/ERK通路促进肿瘤生长，抗体特异性经siRNA敲低实验确认。
3. **"Tissue-specific expression profiling of Rps6ka1 in metabolic regulation using antibody-based detection"**
*Author: Kim H, et al. (2019)*
*摘要：* 研究利用定制的小鼠Rps6ka1抗体，系统分析了RSK1在肝脏、脂肪等代谢相关组织中的表达差异，揭示其与能量代谢的关联，抗体特异性经基因敲除小鼠组织验证。
4. **"Comparative validation of commercial Rps6ka1 antibodies for murine studies"**
*Author: Brown K, et al. (2021)*
*摘要：* 对比了5种商业来源的小鼠Rps6ka1抗体，通过免疫沉淀和免疫荧光实验评估其灵敏度与特异性，推荐了适用于不同实验场景(如冰冻切片/培养细胞)的优选抗体。
注：以上为模拟参考文献，实际文献需通过PubMed/Google Scholar检索确认。
The ribosomal protein S6 kinase A1 (RPS6KA1), also known as p90RSK or RSK1. is a serine/threonine kinase belonging to the RSK family. It plays a critical role in the mitogen-activated protein kinase (MAPK) signaling pathway, mediating cellular responses to growth factors, stress, and hormonal signals. RPS6KA1 is activated via phosphorylation by ERK1/2 and regulates diverse processes, including cell proliferation, survival, differentiation, and gene expression by phosphorylating downstream targets like CREB, c-Fos, and histone H3. In mice, Rps6ka1 is widely expressed and shares high homology with the human ortholog, making it a valuable model for studying its physiological and pathological roles.
Anti-Rps6ka1 antibodies are essential tools for detecting and quantifying this kinase in murine systems. These antibodies are typically developed using immunogenic peptides or recombinant proteins corresponding to specific regions of mouse Rps6ka1. They are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). High-quality antibodies exhibit specificity confirmed by knockout validation or siRNA-mediated silencing. Researchers utilize these antibodies to investigate Rps6ka1's involvement in MAPK/ERK signaling, its crosstalk with other pathways (e.g., mTOR), and its implications in diseases such as cancer, neurological disorders, and metabolic syndromes. Their reliability in distinguishing phosphorylated/activated forms (e.g., via anti-phospho-specific antibodies) further supports mechanistic studies in cellular and tissue contexts.
×
