| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lysine-specific demethylase 6A, 11411-, Histone demethylase UTX, Ubiquitously transcribed TPR protein on the X chromosome, Ubiquitously transcribed X chromosome tetratricopeptide repeat protein, Kdm6a, Utx |
| Entrez GeneID | 22289 |
| WB Predicted band size | 154.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This mouse Kdm6a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1041-1074 amino acids from the C-terminal region of mouse Kdm6a. |
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以下是3-4篇与Mouse Kdm6a抗体相关的参考文献及其简要摘要：
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1. **文献名称**: *UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development*
**作者**: Lee, M.G. et al.
**摘要**: 该研究首次鉴定了Kdm6a(UTX)作为H3K27me2/me3的特异性去甲基化酶，并利用小鼠模型证明其在胚胎发育中的关键作用。研究中通过抗体验证了Kdm6a在小鼠胚胎干细胞中的表达及染色质定位。
2. **文献名称**: *The histone H3K27 demethylase Utx is dispensable for embryonic stem cell differentiation and viability*
**作者**: Shpargel, K.B. et al.
**摘要**: 文章利用Kdm6a基因敲除小鼠模型，结合特异性抗体进行Western blot和免疫组化分析，发现Utx在小鼠胚胎干细胞分化中并非必需，但可能与生殖细胞发育相关。
3. **文献名称**: *Kdm6a regulates the spatial distribution of H3K27me3 and transcriptional identity during mouse germ cell development*
**作者**: Wang, S.P. et al.
**摘要**: 通过抗Kdm6a抗体进行ChIP-seq和免疫荧光染色，揭示了Kdm6a在小鼠生殖细胞中调控H3K27me3修饰的空间分布，影响基因表达谱和细胞命运决定。
4. **文献名称**: *UTX maintains the functional integrity of the murine hematopoietic system through epigenetic regulation*
**作者**: Shinkai, Y. et al.
**摘要**: 研究利用Kdm6a条件性敲除小鼠和特异性抗体，证明UTX通过调控造血干细胞的表观遗传状态维持其功能，缺失会导致小鼠造血系统异常。
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以上文献均涉及Mouse Kdm6a抗体的实验应用(如Western blot、ChIP-seq、免疫组化等)，并聚焦于其在发育、表观遗传调控及疾病中的功能。具体抗体信息可参考原文“Methods”部分或补充材料。
The mouse KDM6A antibody is a crucial tool for studying the KDM6A protein, also known as UTX (ubiquitously transcribed tetratricopeptide repeat protein on the X chromosome), which belongs to the KDM6 family of histone demethylases. KDM6A specifically catalyzes the removal of repressive histone H3 lysine 27 trimethylation (H3K27me3), a modification associated with transcriptional silencing. This enzyme plays a pivotal role in regulating gene expression during development, differentiation, and cellular homeostasis by counteracting Polycomb repressive complex (PRC2)-mediated repression. Dysregulation of KDM6A is linked to various cancers, developmental disorders (e.g., Kabuki syndrome), and immune dysfunction.
Mouse KDM6A antibodies are widely used in research to detect and quantify KDM6A protein levels in murine models, enabling investigations into its tissue-specific expression, subcellular localization, and interaction partners. These antibodies are essential for techniques like Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence. Given KDM6A’s X-chromosome localization and partial functional redundancy with its Y-chromosome homolog UTY, mouse models and corresponding antibodies are critical for dissecting sex-specific roles in disease and development. Validation of antibody specificity (e.g., via knockout controls) is crucial due to potential cross-reactivity with UTY or other demethylases. Research using these antibodies has advanced understanding of KDM6A’s tumor-suppressive roles, its involvement in epigenetic reprogramming, and its therapeutic potential in targeting H3K27me3-related pathologies.
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