| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase RING2, 632-, RING finger protein 1B, RING1b, RING finger protein 2, Rnf2, DinG, Ring1b |
| Entrez GeneID | 19821 |
| WB Predicted band size | 37.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This mouse Rnf2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 165-199 amino acids from the Central region of mouse Rnf2. |
+ +
以下是3篇与小鼠RNF2抗体相关的文献摘要示例(文献信息为虚构，仅供格式参考)：
1. **文献名称**: *RNF2-mediated H2A ubiquitination regulates neural stem cell differentiation in mice*
**作者**: Li X et al.
**摘要**: 本研究利用RNF2特异性抗体，通过ChIP-seq分析发现RNF2在小鼠神经干细胞中通过泛素化H2A抑制分化相关基因，维持干细胞自我更新。抗体验证采用Rnf2基因敲除小鼠脑组织进行Western blot特异性检测。
2. **文献名称**: *RNF2 is essential for PRC1-dependent gene silencing in embryonic development*
**作者**: Wang Y et al.
**摘要**: 作者通过免疫共沉淀(Co-IP)结合RNF2抗体，证明小鼠胚胎中RNF2与PRC1复合物协同作用，调控Hox基因沉默。抗体在E12.5胚胎组织切片中成功用于免疫荧光染色，定位RNF2的核内分布。
3. **文献名称**: *Antibody-based profiling of RNF2 reveals its oncogenic role in murine leukemia models*
**作者**: Tanaka K et al.
**摘要**: 研究利用商业化抗小鼠RNF2抗体，在AML小鼠模型中揭示RNF2通过稳定c-Myc蛋白促进白血病进展。抗体应用于流式细胞术和IHC，证实RNF2在癌变造血细胞中的高表达。
---
注：以上为模拟摘要，实际文献需通过PubMed/Google Scholar以“Rnf2 antibody”、“PRC1 mouse”等关键词检索，推荐筛选抗体验证实验(如KO验证、应用场景明确)的研究。
The RNF2 (Ring Finger Protein 2) antibody is a key tool for studying the Polycomb Repressive Complex 1 (PRC1), a critical regulator of epigenetic gene silencing. RNF2. also known as Ring1B, is a core catalytic subunit of PRC1 and mediates the monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a hallmark of transcriptionally repressive chromatin. This post-translational modification facilitates chromatin compaction and suppresses target gene expression, playing essential roles in embryonic development, stem cell maintenance, and cellular differentiation. Dysregulation of RNF2/PRC1 activity is implicated in cancers, neurodegenerative disorders, and developmental defects.
Antibodies targeting RNF2 are widely used in techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to assess its expression, localization, and DNA-binding activity. They help identify PRC1-mediated gene repression mechanisms and evaluate RNF2's interaction with other Polycomb group proteins (e.g., BMI1. PHC1). Validated RNF2 antibodies are critical for distinguishing its function from its homolog RING1A (Ring1A), as both share overlapping roles but exhibit distinct biological contexts.
Research using RNF2 antibodies has revealed its role in maintaining pluripotency in embryonic stem cells, suppressing tumor suppressor genes in cancers, and regulating X-chromosome inactivation. These studies underscore its potential as a therapeutic target for diseases linked to epigenetic dysregulation. Proper antibody validation via knockout controls or siRNA knockdown is essential to ensure specificity, given the high homology among Ring finger family proteins.
×
