Rabbit Polyclonal NPY2R(N-term) Antibody

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     All lanes : Anti-NPY2R Antibody (N-term) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Anti-NPY2R Antibody (N-term) at  1:1000 dilution + human kidney lysates Lysates/proteins at 20 µg per lane.   Secondary Goat Anti-Rabbit IgG,   (H+L),  Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing SH-SY5Y cells stained with P33049 (green line).  The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( ,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P33049 staining NPY2R in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The NPY2R (N-term) antibody is a targeted immunological tool used to detect the N-terminal region of the Neuropeptide Y Receptor Y2 (NPY2R), a G protein-coupled receptor (GPCR) that binds neuropeptide Y (NPY) and peptide YY (PYY). NPY2R is predominantly expressed in the central nervous system, particularly in hypothalamic regions involved in appetite regulation, as well as in peripheral tissues like the gastrointestinal tract. It plays a critical role in modulating energy homeostasis, feeding behavior, and anxiolytic responses. The N-terminal extracellular domain of NPY2R is essential for ligand binding and receptor activation, making it a key focus for studying receptor-ligand interactions and signaling mechanisms.

This antibody is commonly utilized in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate NPY2R expression, localization, and regulation in various physiological and pathological contexts. Researchers employ it to explore conditions like obesity, metabolic disorders, and stress-related behaviors, where NPY2R signaling is dysregulated. Validation of the antibody typically includes testing in cell lines or tissues with known NPY2R expression and verifying specificity through knockout controls or competitive assays. Its application has advanced understanding of NPY2R's role in neural circuits and potential therapeutic targeting for metabolic and neurological diseases. Proper experimental optimization, such as antigen retrieval in IHC, is often required for optimal results.