| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vasopressin V2 receptor, V2R, AVPR V2, Antidiuretic hormone receptor, Renal-type arginine vasopressin receptor, AVPR2, ADHR, DIR, DIR3, V2R |
| Entrez GeneID | 554 |
| WB Predicted band size | 40.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AVPR2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 343-377 amino acids from the C-terminal region of human AVPR2. |
+ +
以下是3篇关于AVPR2抗体的代表性文献摘要：
1. **文献名称**：Mutations in the vasopressin V2 receptor gene (AVPR2) associated with nephrogenic diabetes insipidus
**作者**：Fujiwara TM, Bichet DG
**摘要**：该研究分析了X连锁肾源性尿崩症患者的AVPR2基因突变，发现多种突变导致受体功能异常，并利用特异性抗体验证突变受体在细胞膜的表达缺陷，揭示突变与疾病表型的关联。
2. **文献名称**：Localization of the human V2 vasopressin receptor gene to Xq28 using a novel allele-specific polymerase chain reaction method and specific antibody labeling
**作者**：Sadeghi H, Inaba T
**摘要**：研究开发了针对AVPR2受体的特异性多克隆抗体，通过免疫荧光和Western blot技术验证抗体特异性，并应用于患者肾组织样本分析，证实抗体在受体定位研究中的有效性。
3. **文献名称**：Molecular cloning and functional characterization of the human V2 vasopressin receptor
**作者**：Thibonnier M, Auzan C
**摘要**：首次报道人源AVPR2受体的分子克隆，通过表达载体在哺乳动物细胞中重组表达，并利用定制抗体进行受体结合实验，阐明其与精氨酸加压素的结合特性及信号转导机制。
The arginine vasopressin receptor 2 (AVPR2) is a G protein-coupled receptor (GPCR) primarily expressed in the kidney’s collecting duct cells. It plays a critical role in regulating water reabsorption by binding to antidiuretic hormone (vasopressin), thereby maintaining body fluid homeostasis. Mutations in the AVPR2 gene are linked to X-linked nephrogenic diabetes insipidus (NDI), a condition characterized by the kidney’s inability to concentrate urine.
AVPR2 antibodies are essential tools for studying receptor expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect AVPR2 in tissue samples or cell lines. Monoclonal antibodies offer high specificity, while polyclonal versions may provide broader epitope recognition. Researchers employ AVPR2 antibodies to investigate receptor dysregulation in diseases like NDI, heart failure, or hypertension, as well as to explore therapeutic interventions targeting vasopressin signaling pathways.
The development of reliable AVPR2 antibodies has advanced understanding of renal physiology and pathophysiology. They also serve as diagnostic aids in confirming genetic mutations associated with NDI. However, challenges remain, including ensuring antibody specificity due to structural similarities among GPCR family members. Validated antibodies are crucial for accurate experimental outcomes in both basic research and clinical applications.
×
