| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEDD4-like E3 ubiquitin-protein ligase WWP2, 632-, Atrophin-1-interacting protein 2, AIP2, WW domain-containing protein 2, WWP2 |
| Entrez GeneID | 11060 |
| WB Predicted band size | 98.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This WWP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 218-252 amino acids from the N-terminal region of human WWP2. |
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以下是关于WWP2 (N-terminal)抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: *WWP2 regulates osteoblast differentiation via PTEN/PI3K/Akt signaling pathway*
**作者**: Chen J, et al.
**摘要**: 本研究利用WWP2 (N-term)抗体检测成骨细胞分化过程中WWP2蛋白的N端表达变化，发现WWP2通过调控PTEN/PI3K/Akt通路抑制成骨分化，抗体特异性经siRNA敲低实验验证。
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2. **文献名称**: *WWP2 interacts with p53 to modulate its function in colorectal cancer*
**作者**: Wang L, et al.
**摘要**: 文章通过免疫共沉淀(Co-IP)结合WWP2 (N-term)抗体，证实WWP2的N端结构域与p53直接结合，并促进p53的泛素化降解，从而影响结直肠癌细胞的增殖和凋亡抵抗。
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3. **文献名称**: *Targeting WWP2 in HER2-positive breast cancer reveals a therapeutic vulnerability*
**作者**: Zhang Y, et al.
**摘要**: 研究使用WWP2 (N-term)抗体进行免疫组化分析，发现HER2阳性乳腺癌组织中WWP2高表达，并通过抑制WWP2降低肿瘤生长，提示其作为潜在治疗靶点。
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**备注**：以上文献为示例性内容，实际文献需通过PubMed或Web of Science等平台检索。若需具体文献DOI或发表年份，建议补充关键词进一步筛选。
The WWP2 (N-term) antibody is a targeted immunological tool designed to detect the N-terminal region of the WW domain-containing E3 ubiquitin protein ligase 2 (WWP2), a member of the NEDD4 family of HECT-type E3 ubiquitin ligases. WWP2 plays a critical role in regulating protein ubiquitination, a post-translational modification essential for protein degradation, localization, and signaling. The enzyme contains multiple functional domains, including an N-terminal C2 domain (involved in membrane binding), four WW domains (mediating protein-protein interactions), and a C-terminal HECT domain (responsible for ubiquitin transfer). The N-terminal region targeted by this antibody is crucial for substrate recognition and cellular localization, enabling WWP2 to interact with diverse signaling molecules such as PTEN, SMADs, and RUNX2. thereby influencing pathways like TGF-β/BMP signaling, osteogenesis, and tumor suppression.
WWP2 is implicated in various diseases, including cancers, where it can act as both an oncogene and tumor suppressor depending on cellular context. The WWP2 (N-term) antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study WWP2 expression, isoform-specific functions (e.g., full-length vs. shorter variants), and regulatory mechanisms. Its specificity for the N-terminal region helps distinguish WWP2 from closely related family members (e.g., WWP1). However, users should validate its performance in their experimental systems, as cross-reactivity or isoform detection may vary. Research employing this antibody has advanced understanding of WWP2’s roles in development, homeostasis, and disease pathogenesis.
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