| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5-hydroxytryptamine receptor 5A, 5-HT-5, 5-HT-5A, 5-HT5A, Serotonin receptor 5A, HTR5A |
| Entrez GeneID | 3361 |
| WB Predicted band size | 40.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This HTR5A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 265-300 amino acids from the Central region of human HTR5A. |
+ +
以下是3-4条关于HTR5A抗体的示例参考文献(内容为虚构，仅供格式参考)：
1. **文献名称**: *"Localization of 5-HT5A receptor in the human brain using a specific antibody"*
**作者**: Thomas, R. et al. (2018)
**摘要**: 该研究通过免疫组化和Western blot技术，利用HTR5A特异性抗体揭示了该受体在人脑前额叶皮层和海马体的高表达，提示其可能在认知和情绪调节中发挥作用。
2. **文献名称**: *"Validation of a novel anti-HTR5A antibody for flow cytometry applications"*
**作者**: Smith, J. & Lee, K. (2019)
**摘要**: 研究验证了一种新型HTR5A抗体的特异性，通过基因敲除细胞系和竞争性结合实验证明其适用于流式细胞术，为受体表面表达定量提供了可靠工具。
3. **文献名称**: *"HTR5A receptor expression in depression models and antidepressant response"*
**作者**: Zhang, Y. et al. (2020)
**摘要**: 利用HTR5A抗体检测慢性应激小鼠模型中的受体表达变化，发现前额叶HTR5A下调与抗抑郁药物反应相关，提示其作为治疗靶点的潜力。
4. **文献名称**: *"Cross-reactivity analysis of commercial HTR5A antibodies in non-human primates"*
**作者**: Patel, S. et al. (2022)
**摘要**: 评估了市售HTR5A抗体在猕猴脑组织中的交叉反应性，发现部分抗体存在非特异性结合，强调了抗体选择对跨物种研究的重要性。
(注：以上文献为示例，实际引用请通过PubMed或Google Scholar查询真实文献。)
The HTR5A antibody targets the 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), a G protein-coupled receptor (GPCR) involved in modulating neurotransmitter signaling. HTR5A is primarily expressed in the central nervous system, particularly in regions like the hippocampus, cortex, and amygdala, where it influences mood regulation, cognition, and circadian rhythms. Unlike other serotonin receptors, HTR5A's function remains less understood, though studies suggest roles in neuropsychiatric disorders such as depression, anxiety, and schizophrenia.
HTR5A antibodies are essential tools for detecting and studying the receptor's expression, localization, and interaction partners. These antibodies are typically developed in animal hosts (e.g., rabbits, mice) using immunogenic peptides derived from specific HTR5A epitopes. They are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize receptor distribution in tissues or cultured cells.
Research using HTR5A antibodies has highlighted its potential as a therapeutic target. For example, selective ligands modulating HTR5A activity are being explored for treating mood disorders. However, challenges persist, including antibody specificity due to structural similarities among serotonin receptor subtypes. Variations in antibody performance across commercial sources also emphasize the need for rigorous validation in experimental models. Overall, HTR5A antibodies remain critical for unraveling the receptor's physiological and pathological roles in neural circuits.
×
