Rabbit Polyclonal MouseNkx2-5 Antibody

The Mouse Nkx2-5 antibody is a critical tool for studying cardiac development and congenital heart diseases. Nkx2-5 (NK2 homeobox 5) is a transcription factor belonging to the NK-2 family, essential for early heart formation, including cardiac progenitor cell specification, heart tube morphogenesis, and chamber septation. In mice, Nkx2-5 is expressed in cardiac precursor cells and persists in adult cardiomyocytes, regulating genes involved in contractility and electrical conduction. Antibodies targeting mouse Nkx2-5 are widely used in techniques like immunohistochemistry, Western blot, and flow cytometry to visualize protein localization, expression levels, or interactions in cardiac tissues or stem cell-derived models. These antibodies typically recognize conserved epitopes, such as the homeodomain responsible for DNA binding, ensuring specificity across species. Researchers employ them to investigate congenital heart defects (e.g., septal abnormalities, conduction disorders) linked to Nkx2-5 mutations and to explore cardiac regeneration or disease mechanisms. Validations often include knockout controls to confirm absence of signal in Nkx2-5-deficient samples. Studies using this antibody have advanced understanding of heart development pathways, including interactions with GATA4. Tbx5. and other core cardiac transcription factors, as well as their roles in pathological remodeling or arrhythmias. Its utility extends to evaluating cardiac differentiation in pluripotent stem cells, aiding disease modeling and therapeutic discovery.