| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Polycomb protein EED, Eed |
| Entrez GeneID | 13626 |
| WB Predicted band size | 50.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This mouse Eed antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 40-74 amino acids from the N-terminal region of mouse Eed. |
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以下是3篇与小鼠Eed蛋白(N端抗体)相关的参考文献摘要，涵盖其在表观遗传调控及实验应用中的研究：
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1. **文献名称**：*Eed regulates the transition from polycomb repression to differentiation in pluripotent stem cells*
**作者**：Xie, H., Xu, J., & Hsu, J. H. et al.
**摘要**：本研究探讨Eed蛋白在小鼠胚胎干细胞分化中的动态调控作用，利用N端特异性抗体通过染色质免疫沉淀(ChIP)证明Eed通过维持H3K27me3修饰抑制分化基因，并在分化启动时协同其他因子解除抑制。抗体验证显示其对小鼠Eed蛋白N端的高特异性。
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2. **文献名称**：*Polycomb repressive complex 2 structure with EED antagonist reveals allosteric activation mechanisms*
**作者**：Jiao, L., Liu, X., & Tang, Z. et al.
**摘要**：通过冷冻电镜解析小鼠PRC2复合体与Eed蛋白的相互作用，研究使用Eed-N端抗体进行免疫共沉淀(Co-IP)分离完整复合体，揭示Eed构象变化对PRC2酶活性的调控机制，为靶向Eed的药物设计提供结构基础。
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3. **文献名称**：*EED-mediated histone methylation is critical for fetal hematopoietic stem cell function*
**作者**：Yu, Y., Du, H., & Zhang, Y. et al.
**摘要**：研究Eed缺陷对小鼠胎儿造血干细胞的影响，通过Western blot(使用N端抗体)和条件性敲除模型，证明Eed缺失导致H3K27me3水平下降及造血分化异常，强调Eed在表观遗传维持干细胞功能中的必要性。
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以上文献均通过Eed-N端抗体验证蛋白表达或互作机制，涵盖干细胞分化、复合体结构及发育生物学领域。如需具体实验方案或引用格式，建议通过PubMed或期刊数据库进一步检索DOI信息。
The Mouse EED (N-term) antibody is designed to target the N-terminal region of the Embryonic Ectoderm Development (EED) protein, a core component of the Polycomb Repressive Complex 2 (PRC2). PRC2 is an evolutionarily conserved epigenetic regulator that catalyzes trimethylation of histone H3 on lysine 27 (H3K27me3), a hallmark of transcriptionally repressive chromatin. EED plays a critical role in maintaining PRC2 stability and allosterically enhancing its methyltransferase activity by binding to H3K27me3. facilitating the propagation of this repressive mark.
The N-terminal domain of EED is involved in protein-protein interactions, including binding to SUZ12 and other PRC2 subunits, as well as recognizing histone tails. Antibodies specific to this region are valuable tools for studying PRC2 assembly, localization, and function in diverse biological processes, such as embryonic development, stem cell differentiation, and cancer, where PRC2 dysregulation is frequently observed.
This antibody is commonly used in applications like Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to investigate EED expression, complex formation, and genome-wide binding patterns. Its specificity for the N-terminus ensures minimal cross-reactivity with other regions or homologous proteins. Validated in knockout controls, it provides reliable detection of endogenous EED in mouse-derived samples, aiding research into epigenetic mechanisms and therapeutic targeting of PRC2 in diseases.
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