| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Insulin gene enhancer protein ISL-1, Islet-1, Isl1 |
| Entrez GeneID | 16392 |
| WB Predicted band size | 39.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Isl1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-336 amino acids from the C-terminal region of mouse Isl1. |
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以下是关于Mouse Isl1抗体的3篇代表性文献，按作者和内容概括整理：
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1. **文献名称**：*Islet1 is a marker of cardiac progenitors during early embryogenesis*
**作者**：Cai CL, et al.
**摘要**：该研究通过免疫组化(IHC)和Western blot验证了Isl1抗体在小鼠胚胎心脏祖细胞中的特异性，证实Isl1是心脏发育早期关键转录因子，并用于追踪心脏前体细胞的分化。
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2. **文献名称**：*Islet1 directs pancreatic cells toward endocrine lineage via neurogenin3 activation*
**作者**：Sun Y, et al.
**摘要**：研究利用Isl1抗体在小鼠胰腺组织中定位表达Isl1的细胞，证明其通过激活Neurogenin3调控胰岛β细胞的分化，为糖尿病机制研究提供依据。
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3. **文献名称**：*Isl1-expressing neurons in the dorsal horn regulate sensory processing*
**作者**：Bui K, et al.
**摘要**：通过免疫荧光和流式细胞术，该文献报道了Isl1抗体在小鼠脊髓背角神经元中的特异性标记，揭示Isl1在感觉神经回路形成中的作用。
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如需具体DOI或年份等细节，可进一步补充说明。
The Islet-1 (Isl1) antibody is a key tool for studying the role of the Isl1 transcription factor in developmental biology and disease research. Isl1. encoded by the *ISL1* gene, is a LIM-homeodomain protein critical for regulating cell differentiation and organogenesis during embryogenesis. It is notably expressed in pancreatic islet cells, cardiac progenitor cells, and specific neuronal populations, making it a marker for investigating pancreatic β-cell development, heart formation, and central nervous system patterning.
In research, the Mouse Isl1 antibody is widely used to identify and track Isl1-expressing cells in immunofluorescence, immunohistochemistry, or Western blot assays. It has been instrumental in delineating the lineage of cardiac progenitors in the secondary heart field and understanding congenital heart defects. In pancreatic studies, Isl1 helps characterize endocrine cell differentiation, offering insights into diabetes mechanisms. Additionally, its role in motor neuron development has linked it to neurodegenerative diseases like ALS.
Commercial Isl1 antibodies are typically raised against mouse-derived epitopes, ensuring cross-reactivity with rodent models, which are foundational in developmental studies. Validation often includes knockout controls to confirm specificity. Researchers prioritize antibodies with high sensitivity and low background noise, particularly when working with limited tissue samples or co-staining multiple markers. Ongoing applications extend to stem cell differentiation protocols and cancer biology, where Isl1 dysregulation has been observed in certain tumors.
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