Rabbit Polyclonal MouseMyocd Antibody

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     Western blot analysis of lysate from mouse C2C12 cell line,  using Myocd Antibody (C-term)(Cat.  #P32894).  P32894 was diluted at 1:1000.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.    

The Mouse Myocd antibody is a crucial tool for studying the role of myocardin (Myocd), a transcriptional coactivator that regulates smooth muscle and cardiac muscle differentiation. Myocd interacts with serum response factor (SRF) to drive the expression of contractile and cytoskeletal genes by binding to CArG box DNA elements. As a master regulator of smooth muscle cell (SMC) identity, Myocd is essential during embryonic development and in maintaining vascular homeostasis. Its dysregulation is implicated in cardiovascular diseases, including atherosclerosis, hypertension, and aortic aneurysms, as well as cancer progression due to its role in modulating cell differentiation and proliferation.

Mouse Myocd antibodies are typically generated against specific epitopes of the murine Myocd protein, enabling detection and localization in tissues or cell lysates via techniques like Western blotting, immunohistochemistry, or immunofluorescence. These antibodies help researchers investigate Myocd expression patterns, its interaction with SRF, and its regulatory mechanisms in disease models. Since Myocd lacks intrinsic DNA-binding ability, its function relies on SRF, making co-localization studies particularly valuable. Mouse-specific antibodies are critical for preclinical studies using murine models to explore therapeutic strategies targeting Myocd pathways. Validation includes testing for specificity in Myocd knockout controls to ensure minimal cross-reactivity with related proteins. Overall, these antibodies advance our understanding of SMC plasticity and Myocd's dual role in health and disease.