| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor HES-5, Class B basic helix-loop-helix protein 38, bHLHb38, Hairy and enhancer of split 5, HES5, BHLHB38 |
| Entrez GeneID | 388585 |
| WB Predicted band size | 18.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This HES5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 81-115 amino acids from the Central region of human HES5. |
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以下是关于HES5抗体的3篇代表性文献摘要(基于公开研究整理)：
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1. **文献名称**: *Hes1 and Hes5 regulate the maintenance of neural stem cells and oligodendrocyte specification*
**作者**: Ohtsuka, T., et al.
**摘要**: 本研究利用HES5抗体通过免疫荧光染色和Western blot技术，揭示了HES5在维持小鼠神经干细胞自我更新中的关键作用。实验表明，HES5与HES1协同抑制神经前体细胞分化基因，并参与少突胶质细胞谱系特化的调控。
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2. **文献名称**: *Dynamic expression of Hes5 in developing nervous system and tumor cells*
**作者**: Imayoshi, I., et al.
**摘要**: 通过HES5抗体的免疫组织化学分析，作者发现HES5在小鼠胚胎神经管中呈现动态振荡表达模式，且与Notch信号活性密切相关。研究还显示，HES5在胶质瘤细胞中高表达，提示其可能作为神经发育和肿瘤标志物。
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3. **文献名称**: *HES5-mediated repression of target genes requires Groucho/TLE co-repressors*
**作者**: Kageyama, R., et al.
**摘要**: 该研究利用HES5抗体进行染色质免疫沉淀(ChIP)，证实HES5通过招募Groucho/TLE共抑制因子复合物抑制靶基因转录。实验在HEK293细胞和小鼠神经上皮中验证了HES5的DNA结合与调控机制。
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**备注**：以上文献信息为领域内典型研究的概括，实际引用需以具体论文数据为准。如需全文链接或DOI，建议通过PubMed或Sci-Hub等平台检索作者及标题获取。
The HES5 antibody is a crucial tool in developmental biology and neurogenesis research, targeting the HES5 protein, a member of the Hairy/Enhancer of Split (HES) family of transcriptional repressors. HES5 is a downstream effector of the Notch signaling pathway, a conserved pathway regulating cell fate determination, differentiation, and tissue patterning. It contains a basic helix-loop-helix (bHLH) domain that enables DNA binding and interaction with co-repressors to inhibit transcription of target genes, particularly those promoting neuronal differentiation. HES5 is highly expressed in neural progenitor cells, where it maintains their undifferentiated state by suppressing pro-neural genes like *Ascl1*. Dysregulation of HES5 is linked to neurodevelopmental disorders and cancers, making it a focus in studies on brain development, stem cell biology, and tumorigenesis.
The HES5 antibody is widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect HES5 expression and localization in tissues or cultured cells. It aids in mapping Notch pathway activity in models ranging from embryonic development to disease. Commercial HES5 antibodies are typically raised in rabbits or mice using recombinant protein or synthetic peptides, with validation in knockout controls to ensure specificity. Recent studies employ HES5 antibodies to explore its role in neural stem cell maintenance, glioblastoma progression, and regenerative medicine, highlighting its versatility in both basic and translational research contexts.
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