Rabbit Polyclonal (Mouse)Ubtfl1 Antibody

The UBTF (Upstream Binding Transcription Factor) antibody is a crucial tool for studying the role of UBTF, a nucleolar phosphoprotein encoded by the *UBTF* gene in humans. UBTF, also known as UBF1. regulates RNA polymerase I-mediated transcription of ribosomal RNA (rRNA) genes, a process vital for ribosome biogenesis and cellular growth. It binds to the upstream control element of rRNA promoters, facilitating the assembly of transcription complexes. Dysregulation of UBTF is implicated in cancers, neurodegenerative diseases, and ribosomopathies. The UBTF antibody is commonly raised in hosts like rabbits or mice, targeting specific epitopes (e.g., human UBTF amino acid regions). It is widely used in techniques such as Western blotting, immunofluorescence, and ChIP-seq to detect UBTF expression levels, subcellular localization (primarily nucleolar), and DNA-binding activity. Validated applications include investigating UBTF's role in cell proliferation, stress responses, and its interactions with oncogenic signaling pathways. Some antibodies distinguish between UBTF isoforms (UBTF1/UBTF2) or post-translational modifications (e.g., phosphorylation). Researchers often confirm specificity using siRNA knockdown or *UBTF*-null cell lines. Commercial variants may differ in clonality (monoclonal/polyclonal) and conjugates (e.g., HRP, fluorescent tags). Its utility spans cancer biology, developmental studies, and ribosome dysfunction research.