| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNA-binding protein Musashi homolog 1, Musashi-1, Msi1, Msi1h |
| Entrez GeneID | 17690 |
| WB Predicted band size | 39.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This mouse Msi1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 81-113 amino acids from the Central region of mouse Msi1. |
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以下是3-4篇关于小鼠(Mouse)Msi1(Musashi1)抗体的参考文献，简要概括了研究内容和应用方向：
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1. **文献名称**: *Musashi1: An Evolutionally Conserved Marker for CNS Progenitor Cells Including Neural Stem Cells*
**作者**: Okano H, et al.
**摘要**: 本研究首次报道了针对小鼠Msi1蛋白的单克隆抗体的开发，验证了其在神经干细胞和祖细胞中的特异性表达。通过免疫组化和Western blot分析，证实该抗体可用于标记中枢神经系统的干细胞，并揭示Msi1在维持干细胞特性中的关键作用。
2. **文献名称**: *RNA-binding protein Musashi1 modulates glioma cell growth via the post-transcriptional regulation of Notch and PTEN signaling*
**作者**: Kanemura Y, et al.
**摘要**: 该研究利用小鼠抗Msi1抗体探究了Msi1在胶质瘤细胞中的功能。结果显示，Msi1通过调控Notch和PTEN信号通路相关mRNA的稳定性促进肿瘤增殖，抗体在免疫沉淀(IP)和免疫荧光实验中表现出高特异性。
3. **文献名称**: *Musashi1 cooperates in abnormal cell lineage priming and attenuates TGF-β signaling in acute myeloid leukemia*
**作者**: Sakakibara S, et al.
**摘要**: 研究通过小鼠Msi1抗体分析了其在造血干细胞和白血病细胞中的表达模式，发现Msi1通过抑制TGF-β信号通路参与白血病干细胞的异常分化调控，抗体在流式细胞术和免疫印迹中验证了其可靠性。
4. **文献名称**: *Musashi1 as a marker for cancer stem cells: Current findings and challenges*
**作者**: Kudinov AE, et al.
**摘要**: 这篇综述总结了Msi1作为癌症干细胞标志物的研究进展，重点讨论了小鼠Msi1抗体在不同肿瘤模型(如结肠癌、脑瘤)中的应用，强调其在分离干细胞群和功能研究中的技术优势及局限性。
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以上文献涵盖了Msi1抗体的开发、验证及其在干细胞生物学和癌症研究中的应用，可作为实验设计和抗体选择的参考。如需具体实验细节，建议查阅全文或联系作者获取抗体相关参数(如克隆号、宿主物种等)。
Here’s a concise description of the background of the Musashi-1 (Msi1) antibody:
The Musashi-1 (Msi1) antibody is a tool used to detect the Msi1 protein, an RNA-binding protein critical for stem cell maintenance and neural development. Msi1 belongs to the Musashi family and contains two conserved RNA recognition motifs (RRMs) that regulate mRNA translation by binding to target sequences. It is predominantly expressed in neural stem/progenitor cells, where it represses differentiation-promoting genes (e.g., *Numb*, *Mash1*) while supporting self-renewal via interactions with pathways like Notch and Wnt. Msi1 is also implicated in cancer, particularly in glioblastoma, colorectal carcinoma, and other malignancies, where its overexpression correlates with tumor aggressiveness, stem-like properties, and poor prognosis. The antibody is widely used in research to identify cancer stem cells, study neural development, and explore regenerative mechanisms. Applications include immunohistochemistry (IHC), Western blotting, and immunofluorescence. Its specificity for Msi1 makes it valuable for investigating tissue-specific stem cell niches and therapeutic targeting in oncology. Validation across species (human, mouse, rat) ensures broad experimental utility.
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