| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin carboxyl-terminal hydrolase 22, Deubiquitinating enzyme 22, Ubiquitin thioesterase 22, Ubiquitin-specific-processing protease 22, USP22, KIAA1063, USP3L |
| Entrez GeneID | 23326 |
| WB Predicted band size | 60.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This USP22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the C-terminal region of human USP22. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇关于USP22抗体的代表性文献摘要(人工整理，非真实文献，仅作示例)：
1. **《USP22 regulates stem cell pluripotency through histone H2B deubiquitination》**
*作者：Glinsky GV, et al. (2005)*
摘要：研究利用USP22特异性抗体验证其在胚胎干细胞中的表达，发现其通过去泛素化组蛋白H2B调控多能性基因(如OCT4)的转录活性。
2. **《USP22 promotes tumor progression via HIF-1α stabilization in colorectal cancer》**
*作者：Zhang L, et al. (2012)*
摘要：通过Western blot和免疫组化(使用兔源USP22单抗)，揭示USP22在结直肠癌中高表达，并通过稳定HIF-1α促进肿瘤血管生成和转移。
3. **《USP22 as a prognostic biomarker in hepatocellular carcinoma》**
*作者：Lin Y, et al. (2016)*
摘要：采用抗USP22抗体进行组织芯片分析，发现USP22过表达与肝癌患者生存期缩短相关，提示其可作为肝癌预后标志物和治疗靶点。
注：以上文献信息为模拟示例，实际引用需核对真实数据库(如PubMed)。如需真实文献，建议检索关键词"USP22 antibody" + 应用场景(如cancer/IHC/WB)。
The USP22 (Ubiquitin Specific Peptidase 22) antibody is a key tool for studying the USP22 protein, a deubiquitinating enzyme belonging to the ubiquitin-specific protease (USP) family. USP22 plays critical roles in transcriptional regulation, cell cycle progression, and epigenetic modification by removing ubiquitin moieties from target substrates, thereby stabilizing them. It is a core component of the human SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, which regulates histone H2B deubiquitination and gene expression. USP22’s involvement in cancer biology has drawn significant attention, as its overexpression correlates with tumor progression, metastasis, and poor prognosis in various cancers, including colorectal, breast, and liver cancers.
USP22 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to detect USP22 expression levels, subcellular localization, and protein interactions. Researchers rely on these antibodies to explore USP22’s functional mechanisms, such as its role in cancer stem cell maintenance, chemoresistance, and immune evasion. Specificity and validation (e.g., knockout controls) are critical due to potential cross-reactivity with other USP family members. Commercial USP22 antibodies are often raised against peptide sequences unique to the protein’s C- or N-terminal regions. Recent studies also investigate USP22 as a potential therapeutic target, underscoring the antibody’s importance in both basic research and translational applications.
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