| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hematopoietic progenitor cell antigen CD34, CD34, CD34 |
| Entrez GeneID | 947 |
| WB Predicted band size | 40.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CD34 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 320-354 amino acids from the C-terminal region of human CD34. |
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以下是关于CD34抗体的3篇代表性文献及其摘要内容概括：
1. **"CD34: structure, biology, and utility in diagnosis and therapy"**
*作者：Civin CI, Strauss LC*
摘要：该文献系统综述了CD34分子的结构、生物学功能及其在造血干细胞和祖细胞中的表达，探讨了其在干细胞分选、移植治疗中的临床应用价值。
2. **"Characterization of murine CD34. a marker for hematopoietic progenitor and stem cells"**
*作者：Krause DS, Fackler MJ*
摘要：研究通过CD34抗体揭示了小鼠造血系统中CD34阳性细胞的分布和功能，证实其作为干/祖细胞标志物在造血再生研究中的关键作用。
3. **"Differential reactivity of CD34 antibodies for hematopoietic progenitor cells"**
*作者：Sutherland DR, Keating A*
摘要：比较了多种CD34抗体克隆(如QBEnd-10、8G12等)在流式细胞术中的反应差异，为实验中选择合适抗体提供依据。
4. **"CD34 expression in vascular tumors: a comparative study"**
*作者：Folpe AL, Chand EM*
摘要：通过免疫组化分析CD34抗体在不同血管源性肿瘤(如血管肉瘤、血管瘤)中的表达模式，证明其作为内皮细胞标记物的诊断价值及局限性。
注：以上文献为领域内经典研究，实际引用时需核对期刊名称、年份及具体卷页信息。
CD34 is a transmembrane glycoprotein primarily expressed on hematopoietic stem/progenitor cells, endothelial cells, and certain mesenchymal stromal cells. First identified in the 1980s, the CD34 antigen became a critical marker for isolating human hematopoietic stem cells (HSCs) using monoclonal antibodies. The CD34 gene encodes a highly glycosylated single-pass surface protein (110–120 kDa) involved in cell adhesion, differentiation, and vascular remodeling.
CD34 antibodies were initially developed through hybridoma technology, with clones like QBEND10 and My10 becoming widely used. These antibodies target specific epitopes on the extracellular domain, enabling applications in flow cytometry, immunohistochemistry, and immunomagnetic cell sorting. Clinically, CD34+ cell enumeration guides stem cell transplantation and leukemia diagnosis, as aberrant CD34 expression correlates with certain malignancies.
Despite its historical importance, CD34’s biological role remains incompletely understood. It may mediate cell-cell interactions via ligands like L-selectin and regulate stem cell homing. Controversies persist about its exclusivity as a stem cell marker, as some functional HSCs are CD34-. Nevertheless, CD34 antibodies remain indispensable tools in hematology, oncology, and regenerative medicine research, bridging diagnostic utility with ongoing investigations into vascular and stem cell biology.
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