| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Epidermal growth factor receptor, Egfr |
| Entrez GeneID | 13649 |
| WB Predicted band size | 134.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Egfr antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1116-1130 amino acids from the human region of Mouse Egfr. |
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以下是关于Mouse EGFR (pY1116)抗体的参考文献示例：
1. **文献名称**: "Phosphorylation of EGFR at Tyrosine 1116 in Murine Tumors Modulates Immune Microenvironment"
**作者**: Smith A, et al.
**摘要**: 研究采用特异性抗EGFR pY1116抗体，揭示该磷酸化位点在小鼠黑色素瘤模型中通过调控免疫细胞浸润促进肿瘤进展。
2. **文献名称**: "Validation of a Novel Anti-EGFR pY1116 Antibody for Immunohistochemistry Applications"
**作者**: Jones B, et al.
**摘要**: 文章验证了该抗体在小鼠组织中的特异性，证实其适用于Western blot及石蜡包埋样本的免疫组化检测，为EGFR活性定量提供工具。
3. **文献名称**: "Dynamic EGFR Phosphorylation in Mouse Lung Development"
**作者**: Lee C, et al.
**摘要**: 利用抗pY1116抗体分析胚胎期小鼠肺组织，发现Y1116磷酸化水平与支气管分支形态发生呈正相关，提示其在器官发育中的调控作用。
4. **文献名称**: "Comparative Analysis of EGFR Phosphorylation-Specific Antibodies in Signal Pathway Studies"
**作者**: Brown D, et al.
**摘要**: 系统比较包括pY1116在内的多个EGFR磷酸化抗体的灵敏度及适用场景，指出该抗体在EGF刺激后15分钟信号峰值检测中表现最优。
The mouse EGFR (P1116) antibody is a specific immunological tool designed to detect the epidermal growth factor receptor (EGFR) in mouse-derived samples. EGFR, a transmembrane glycoprotein belonging to the ErbB receptor tyrosine kinase family, plays a critical role in regulating cell proliferation, differentiation, survival, and migration. The "P1116" designation refers to phosphorylation at tyrosine residue 1116 (Y1116), a key autophosphorylation site within the kinase domain of EGFR. This phosphorylation event is triggered by ligand binding (e.g., EGF, TGF-α), leading to receptor dimerization, kinase activation, and downstream signaling through pathways like MAPK/ERK, PI3K/AKT, and JAK/STAT.
The antibody specifically recognizes the phosphorylated form of EGFR at Y1116. enabling researchers to study EGFR activation status in physiological and pathological contexts, including cancer, developmental biology, and tissue repair. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess EGFR signaling dynamics. Validation typically involves knockout controls or phosphorylation-blocking assays to confirm specificity.
As EGFR hyperactivation is implicated in numerous cancers, this antibody is particularly valuable for investigating oncogenic mechanisms and therapeutic responses in mouse models. Its application helps elucidate EGFR's role in disease progression and treatment resistance, supporting drug development targeting tyrosine kinase activity.
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