关键信息
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基因名
LPCAT2
- 应用
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别名
LPCAT2；AGPAT11；AYTL1；Lysophosphatidylcholine acyltransferase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7L5N7
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表达区间
1-544aa
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氨基酸序列
MSRCAQAAEVAATVPGAGVGNVGLRPPMVPRQASFFPPPVPNPFVQQTQIGSARRVQIVLLGIILLPIRVLLVALILLLAWPFAAISTVCCPEKLTHPITGWRRKITQTALKFLGRAMFFSMGFIVAVKGKIASPLEAPVFVAAPHSTFFDGIACVVAGLPSMVSRNENAQVPLIGRLLRAVQPVLVSRVDPDSRKNTINEIIKRTTSGGEWPQILVFPEGTCTNRSCLITFKPGAFIPGVPVQPVLLRYPNKLDTVTWTWQGYTFIQLCMLTFCQLFTKVEVEFMPVQVPNDEEKNDPVLFANKVRNLMAEALGIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFTKISRKLKLDWDGVRKHLDEYASIASSSKGGRIGIEEFAKYLKLPVSDVLRQLFALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYITEEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKIFTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPEKHEESTSDKKDD
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分子量
60.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
LPCAT2 (Lysophosphatidylcholine acyltransferase 2) is a crucial enzyme involved in lipid metabolism, specifically in the biosynthesis of phosphatidylcholine, a key component of cell membranes. Recent studies have illuminated the role of LPCAT2 in various physiological and pathological processes, including inflammation, cancer, and metabolic disorders. Given its significance in maintaining cellular lipid homeostasis, LPCAT2 has attracted interest as a potential therapeutic target for diseases like non-alcoholic fatty liver disease (NAFLD) and atherosclerosis. Research on the recombinant expression of LPCAT2 enables scientists to obtain the protein in purified form for biochemical and structural studies, facilitating a deeper understanding of its enzymatic mechanism and regulatory functions. Advances in protein engineering and expression systems have made it feasible to produce LPCAT2 in sufficient quantities for functional assays and high-resolution structural analysis. This knowledge may lead to the identification of specific inhibitors or modulators of LPCAT2 activity, with the potential for new drug development strategies. Furthermore, studying the interactions of LPCAT2 with other lipid metabolic pathways could provide insights into its broader role in cell signaling and disease progression, underscoring the importance of LPCAT2 in health and disease. Overall, the research on LPCAT2 recombinant protein is vital for elucidating the enzyme's biological functions and its implications in translational medicine.
