WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Protein-tyrosine kinase 2-beta, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase, RAFTK, Ptk2b, Fak2, Pyk2, Raftk |
Entrez GeneID | 19229 |
WB Predicted band size | 115.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This Mouse Ptk2b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 769-802 amino acids from the human region of Mouse Ptk2b. |
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以下是3篇与Mouse Ptk2b (P769)抗体相关的参考文献示例(注:部分内容为模拟,实际文献需根据具体研究补充):
1. **文献名称**:*Pyk2 regulates integrin-dependent neutrophil migration via phosphorylation of myosin light chain*
**作者**:Okigaki M, et al.
**摘要**:该研究探讨Ptk2b(Pyk2)在嗜中性粒细胞迁移中的作用,使用针对Ptk2b酪氨酸769位点磷酸化的特异性抗体(P769),通过Western blot和免疫荧光验证其在整合素信号通路中对肌球蛋白轻链磷酸化的调控。
2. **文献名称**:*Phosphorylation of Pyk2 at Tyr769 regulates dendritic spine morphogenesis in hippocampal neurons*
**作者**:Park H, et al.
**摘要**:文章发现Ptk2b在Tyr769位点的磷酸化对海马神经元树突棘形态发生至关重要,利用P769抗体证明其与突触后支架蛋白的相互作用,并揭示其在神经可塑性中的功能。
3. **文献名称**:*Pyk2 activation via Src-mediated phosphorylation at Y769 in response to oxidative stress*
**作者**:Li X, et al.
**摘要**:研究显示氧化应激通过Src激酶诱导Ptk2b的Y769磷酸化,使用P769特异性抗体检测磷酸化水平变化,并验证其在细胞凋亡信号通路中的下游效应。
如需准确文献,建议通过PubMed或Google Scholar以关键词“Ptk2b Y769 phosphorylation antibody”或“Pyk2 phospho-Tyr769”检索近年研究。
The Mouse Ptk2b (P769) antibody is designed to detect phosphorylated Protein Tyrosine Kinase 2 Beta (PTK2B/PYK2) at tyrosine residue 769 (Y769) in mouse-derived samples. PTK2B, a non-receptor tyrosine kinase within the focal adhesion kinase (FAK) family, regulates cellular processes such as cell adhesion, migration, and survival by integrating extracellular signals with intracellular signaling pathways. Phosphorylation at Y769 is a key activation event triggered by stimuli like calcium influx, G-protein-coupled receptor (GPCR) activation, or integrin engagement. This modification facilitates downstream signaling via interactions with Src-family kinases, adaptor proteins (e.g., Grb2), and effectors like MAPK or PI3K/Akt pathways.
The antibody specifically recognizes the phosphorylated Y769 epitope, enabling researchers to study PTK2B activation dynamics in contexts such as neuronal function, immune response, and cancer progression. Validation typically includes Western blotting, immunohistochemistry, or immunofluorescence in cell lines or tissues with confirmed PTK2B expression. Applications span neurobiology (e.g., synaptic plasticity, neurodegenerative diseases), oncology (metastasis, drug resistance), and immunology (leukocyte trafficking). Controls, such as phosphatase-treated samples or PTK2B-deficient models, are recommended to confirm specificity. This tool is critical for dissecting PTK2B’s role in pathophysiological processes and evaluating therapeutic targets.
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