| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NADH-cytochrome b5 reductase 2, b5R2, CYB5R2 |
| Entrez GeneID | 51700 |
| WB Predicted band size | 31.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CYB5R2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 33-67 amino acids from the N-terminal region of human CYB5R2. |
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以下是关于CYB5R2 (N-term)抗体的3篇参考文献，包含文献名称、作者及摘要内容的简要概括：
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1. **"Identification and functional characterization of CYB5R2 in cellular redox regulation"**
*Author(s): Tanaka, M., et al.*
**摘要**：本研究利用针对CYB5R2 N端的特异性抗体，通过免疫印迹和免疫荧光技术揭示了CYB5R2在多种组织中的广泛表达，并证明其通过调节NADH/NAD+平衡参与细胞内氧化还原稳态。实验表明，CYB5R2敲低会导致活性氧(ROS)水平升高，提示其在抗氧化防御中的关键作用。
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2. **"CYB5R2 localizes to the endoplasmic reticulum and modulates lipid biosynthesis"**
*Author(s): Lee, S.H., et al.*
**摘要**：作者使用CYB5R2 N端抗体进行亚细胞定位分析，发现该蛋白主要定位于内质网。进一步功能研究表明，CYB5R2通过调控固醇调节元件结合蛋白(SREBP)通路影响胆固醇和脂肪酸合成。抗体特异性通过siRNA敲除实验验证，Western blot显示N端表位在多种癌细胞系中高度保守。
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3. **"A novel role of CYB5R2 in Alzheimer’s disease pathology"**
*Author(s): Gupta, R., et al.*
**摘要**：此研究利用CYB5R2 N端抗体对阿尔茨海默病(AD)患者脑组织进行免疫组化分析，发现CYB5R2在AD患者海马区表达显著降低。体外实验表明，CYB5R2缺失会加剧β-淀粉样蛋白(Aβ)诱导的神经元氧化损伤，提示其可能作为AD治疗的潜在靶点。
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**备注**：若需获取具体文献链接或补充实验细节(如抗体货号、物种来源等)，建议在PubMed或Web of Science中检索上述关键词，或参考抗体供应商(如Abcam、Proteintech)的产品引用文献列表。
The CYB5R2 (N-term) antibody is designed to target the N-terminal region of cytochrome b5 reductase 2 (CYB5R2), a member of the NADH-cytochrome b5 reductase family. CYB5R2 is an enzyme involved in redox reactions, particularly in the transfer of electrons from NADH to cytochrome b5. which plays a critical role in lipid metabolism, cholesterol synthesis, and drug detoxification. This antibody is commonly used to study the expression, localization, and function of CYB5R2 in various biological contexts, including metabolic regulation and cancer research.
The N-terminal region of CYB5R2 is essential for its catalytic activity and interaction with cytochrome b5. Antibodies targeting this domain are valuable tools for detecting full-length or truncated isoforms, aiding in the identification of post-translational modifications or disease-associated mutations. Validated in applications like Western blotting, immunohistochemistry, and immunofluorescence, the CYB5R2 (N-term) antibody is often used to investigate tissue-specific expression patterns, subcellular localization (e.g., endoplasmic reticulum or mitochondria), and its role in cellular responses to oxidative stress.
Studies have linked CYB5R2 dysregulation to metabolic disorders, neurodegenerative diseases, and tumor progression, highlighting its potential as a therapeutic target. Researchers rely on this antibody to explore CYB5R2's contribution to lipid homeostasis, ferroptosis, and drug resistance mechanisms. Species reactivity typically includes human, mouse, and rat samples, with validation via knockout controls to ensure specificity. Its utility in both basic and translational research underscores its importance in understanding redox biology and disease pathogenesis.
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