| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger imprinted 2, Zinc finger protein 656, ZIM2, ZNF656 |
| Entrez GeneID | 23619 |
| WB Predicted band size | 61.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZIM2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 39-73 amino acids from the N-terminal region of human ZIM2. |
+ +
以下是关于ZIM2(N-term)抗体的3篇参考文献的简要总结(注：文献信息为模拟示例，实际文献需通过学术数据库检索确认)：
---
1. **文献名称**: *ZIM2-mediated transcriptional regulation in early embryonic development*
**作者**: Smith A, et al.
**摘要**: 本研究利用ZIM2(N-term)抗体(货号AB123)通过免疫沉淀和Western blot技术，验证了ZIM2蛋白在小鼠早期胚胎中的表达模式。实验显示，该抗体特异性识别ZIM2的N端结构域，并在干细胞分化模型中检测到ZIM2与DNA甲基化调控因子的相互作用。
2. **文献名称**: *Epigenetic roles of ZIM2 in human germ cell programming*
**作者**: Chen L, et al.
**摘要**: 研究通过ZIM2(N-term)抗体的免疫组化分析，揭示了ZIM2在人类生殖细胞发育中的动态定位。抗体特异性验证显示，其N端表位在多种哺乳动物中高度保守，且抗体在ChIP-seq实验中成功富集了ZIM2结合的印记基因调控区域。
3. **文献名称**: *Antibody validation for zinc-finger proteins: A case study on ZIM2*
**作者**: Johnson R, et al.
**摘要**: 本文系统评估了多种ZIM2抗体的特异性，包括针对N端区域的商业抗体(如Sigma-Aldrich HPA012345)。通过CRISPR敲除细胞系验证，确认该抗体在免疫荧光和流式细胞术中无交叉反应，适用于研究ZIM2在癌症表观遗传重编程中的功能。
---
**提示**：实际研究中，建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表检索相关文献，并关注抗体验证方法和应用场景的描述。
The ZIM2 (N-term) antibody is designed to detect the N-terminal region of Zinc-finger protein expressed in Inflorescence Meristem 2 (ZIM2), a plant-specific transcription factor involved in various developmental processes. ZIM2 belongs to the ZIM/ ZFP (Zinc-finger Inflorescence Meristem) protein family, which is characterized by tandem C2H2-type zinc-finger domains and plays roles in regulating flowering, seed development, and hormone signaling, particularly in model organisms like *Arabidopsis thaliana*. The N-terminal region of ZIM2 is critical for protein-protein interactions and transcriptional regulation, often mediating associations with chromatin modifiers or other regulatory complexes.
This antibody is commonly used in plant molecular biology to study ZIM2 expression, localization, and function via techniques such as Western blotting, immunoprecipitation, or immunohistochemistry. It helps researchers investigate ZIM2's role in epigenetic regulation, such as its interaction with Polycomb Repressive Complex 2 (PRC2) to modulate gene silencing during seed development. Specificity for the N-terminal epitope ensures minimal cross-reactivity with other ZIM family members (e.g., ZIM1. ZIM3), making it a valuable tool for dissecting isoform-specific functions.
Developed using immunogenic peptides from the N-terminal domain, the antibody is typically validated in knockout mutants or knockdown lines to confirm target specificity. Its applications span studies on plant reproduction, abiotic stress responses, and developmental transitions linked to phytohormones like gibberellins.
×
