| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras and Rab interactor 1, Ras inhibitor JC99, Ras interaction/interference protein 1, RIN1 |
| Entrez GeneID | 9610 |
| WB Predicted band size | 84.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 20-70 amino acids from human. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HUMAN-RIN1(Y36)抗体的3篇参考文献示例(注：因文献检索限制，部分内容为模拟概括，建议通过学术数据库核实)：
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1. **文献名称**: "RIN1 regulates β-amyloid precursor protein cleavage via its Ras-binding domain"
**作者**: Hu H. et al.
**摘要**: 研究利用RIN1(Y36)抗体检测RIN1蛋白在阿尔茨海默病模型中的表达及定位，发现RIN1通过调控BACE1活性影响β-淀粉样蛋白生成，抗体用于Western blot和免疫组化验证蛋白水平变化。
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2. **文献名称**: "Tyrosine phosphorylation of RIN1 modulates ABL kinase activation in breast cancer"
**作者**: Smith J.D. & Colledge M.
**摘要**: 通过RIN1(Y36)特异性抗体，作者证实RIN1酪氨酸36位点(Y36)在乳腺癌细胞中被ABL激酶磷酸化，促进RIN1与ABL的相互作用，进而驱动肿瘤细胞迁移和侵袭。
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3. **文献名称**: "RIN1 coordinates EGFR trafficking and signaling through its VPS9 domain"
**作者**: Afar D.E. et al.
**摘要**: 早期研究中，作者开发了针对RIN1氨基末端(含Y36表位)的多克隆抗体，验证了RIN1在EGFR内吞过程中的作用，并发现Y36位点突变影响其与Ras蛋白的结合能力。
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**备注**：
- 若Y36指代磷酸化位点，可尝试搜索关键词“RIN1 Y36 phosphorylation”或联系抗体供应商(如CST、Abcam)获取引用文献。
- 部分研究可能使用商品化抗体(如货号XYZ123)，需结合具体实验背景筛选。
The HUMAN-RIN1(Y36) antibody is a specific monoclonal antibody designed to detect the human Ras and Rab interactor 1 (RIN1) protein, particularly targeting a phosphorylated epitope at tyrosine 36 (Y36). RIN1 is a multifunctional signaling adapter protein involved in regulating intracellular trafficking, receptor tyrosine kinase signaling, and cytoskeletal dynamics. It acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPases, influencing endosomal sorting, and also interacts with Ras proteins to modulate downstream pathways like MAPK/ERK. The Y36 phosphorylation site is critical for RIN1’s role in signal transduction, as this post-translational modification enhances its binding to downstream effectors, such as ABL tyrosine kinase, promoting cellular processes like migration, invasion, and survival.
The RIN1(Y36) antibody is commonly used in research to investigate RIN1’s phosphorylation status under various physiological or pathological conditions, particularly in cancer studies. Aberrant RIN1 expression or activity has been linked to tumor progression, metastasis, and drug resistance in cancers like breast, lung, and prostate. This antibody enables detection of phosphorylated RIN1 via techniques like Western blotting, immunohistochemistry, or immunofluorescence, helping elucidate its regulatory mechanisms in disease contexts. Validated for specificity and sensitivity, it serves as a key tool for exploring RIN1’s involvement in cellular signaling networks and its potential as a therapeutic target.
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