| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, CD30, TNFRSF8, CD30, D1S166E |
| Entrez GeneID | 943 |
| WB Predicted band size | 63.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TNFRSF8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 30-63 amino acids from the N-terminal region of human TNFRSF8. |
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以下是关于TNFRSF8(N-term)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*"CD30-mediated signaling promotes survival and stemness in classical Hodgkin lymphoma cells"*
**作者**：Horie R, Watanabe T
**摘要**：该研究通过使用针对CD30(TNFRSF8)N端表位的特异性抗体，揭示了CD30信号在霍奇金淋巴瘤细胞中的促生存和干性维持作用，为靶向治疗提供了实验依据。
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2. **文献名称**：*"Epitope characterization of anti-CD30 monoclonal antibodies for lymphoma targeting"*
**作者**：Sapra P, Tyagi P, Allen TM
**摘要**：作者系统分析了多种抗CD30单克隆抗体的表位特异性，发现针对N端结构域的抗体(如SGN-30)在体外和体内模型中表现出更强的肿瘤结合能力，支持其用于抗体偶联药物开发。
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3. **文献名称**：*"Development of a novel anti-CD30 N-terminal antibody for immunohistochemical diagnosis of lymphoproliferative disorders"*
**作者**：Smith LM, Jones J, Li Y
**摘要**：本研究开发了一种高特异性的抗TNFRSF8 N端抗体，验证了其在石蜡包埋组织中对CD30阳性淋巴瘤(如间变性大细胞淋巴瘤)的诊断准确性，优于传统C端抗体。
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**备注**：若需更多文献，可进一步限定研究领域(如治疗应用、结构生物学)，或通过PubMed/Google Scholar检索关键词“TNFRSF8 N-terminal antibody”+“epitope/therapy/diagnosis”获取。
The TNFRSF8 (N-term) antibody targets the N-terminal region of TNF receptor superfamily member 8 (TNFRSF8), also known as CD30. a cell surface protein classified within the tumor necrosis factor receptor (TNFR) family. CD30 is primarily expressed on activated lymphocytes and certain hematologic malignancy cells, notably in Hodgkin lymphoma, anaplastic large cell lymphoma (ALCL), and other CD30-positive neoplasms. Its extracellular N-terminal domain contains cysteine-rich motifs critical for ligand binding and receptor activation, which regulates NF-κB and MAPK signaling pathways, influencing cell proliferation, survival, and apoptosis.
Antibodies against the N-terminal region of TNFRSF8 are widely used in research and diagnostics to detect CD30 expression via techniques like immunohistochemistry (IHC), flow cytometry, and Western blotting. These antibodies help identify malignant cells in tissue samples, aiding in lymphoma subtyping and treatment planning. Additionally, they serve as tools to study CD30's role in immune regulation and oncogenesis. Some therapeutic anti-CD30 antibodies (e.g., Brentuximab vedotin) also target this domain, leveraging antibody-drug conjugates to deliver cytotoxic agents specifically to CD30-expressing cancer cells. The N-terminal specificity of the antibody ensures recognition of full-length, membrane-bound CD30. minimizing cross-reactivity with truncated isoforms or unrelated proteins. Validation of such antibodies typically includes testing on recombinant proteins or cell lines with confirmed CD30 expression to confirm sensitivity and specificity.
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