| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89, znf148, zbp89 |
| Entrez GeneID | 560300 |
| WB Predicted band size | 90.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) znf148 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 287-320 amino acids of DANRE znf148. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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由于当前无法访问实时数据库，以下为假设性参考文献示例，仅供参考。建议通过PubMed或Google Scholar检索实际文献：
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1. **文献名称**: "Zebrafish ZNF148 regulates neural crest development through histone deacetylase interaction"
**作者**: Kim J, et al.
**摘要**: 研究斑马鱼ZNF148在神经嵴细胞分化中的作用，利用特异性抗体进行蛋白定位分析，揭示其通过结合组蛋白去乙酰化酶调控靶基因表达。
2. **文献名称**: "Development and Validation of a Polyclonal Antibody Against Danio rerio ZNF148 for Immunoblotting Applications"
**作者**: Müller S, et al.
**摘要**: 描述抗斑马鱼ZNF148多克隆抗体的制备与验证，证实其在Western blot和免疫荧光中的特异性，为后续功能研究提供工具。
3. **文献名称**: "CRISPR/Cas9 knockout of znf148 in zebrafish reveals its essential role in cardiac valve formation"
**作者**: Chen L, et al.
**摘要**: 通过CRISPR敲除结合抗体免疫染色，证明ZNF148缺失导致斑马鱼心脏瓣膜发育异常，提示其在心血管系统中的关键功能。
4. **文献名称**: "Dynamic expression patterns of ZNF148 during zebrafish embryonic development"
**作者**: Gupta R, et al.
**摘要**: 利用抗体进行时空表达分析，发现ZNF148在斑马鱼胚胎的脑部和体节中阶段性高表达，暗示其参与早期器官发生。
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**备注**：以上文献为示例性质，实际文献需通过学术数据库查询。建议使用关键词“ZNF148 Danio rerio antibody”或“zebrafish znf148”进行检索。
The DANRE (ZNF148) antibody is a research tool designed to detect zinc finger protein 148 (ZNF148), a transcription factor belonging to the Krüppel-associated box (KRAB) zinc finger family. ZNF148. also known as Zfp148 in mice or ZNF148 in humans, plays roles in regulating gene expression, cell cycle progression, and apoptosis. It interacts with DNA and protein partners, such as p53 and SMAD3. influencing pathways implicated in cancer, development, and fibrosis.
Antibodies targeting ZNF148 are commonly produced in hosts like rabbits or mice, using immunogens derived from specific regions of the protein (e.g., N-terminal or C-terminal domains). They are widely used in techniques including Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to study ZNF148's expression, localization, and molecular interactions. Validation often involves knockout cell lines or siRNA-mediated knockdown to confirm specificity.
Commercial ZNF148 antibodies are available from suppliers like Abcam, Santa Cruz Biotechnology, and Cell Signaling Technology. However, reported cross-reactivity or batch variability highlights the need for careful validation in each experimental system. Research using these antibodies has contributed to understanding ZNF148's dual roles in tumor suppression (e.g., colorectal cancer) and oncogenesis (e.g., glioblastoma), underscoring its context-dependent functions.
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