Rabbit Polyclonal (DANRE)hoxb6a Antibody

中文名： (DANRE) hoxb6a抗体
别名： Homeobox protein Hox-B6a, Hox-B6, Homeobox protein Zf-22, hoxb6a, hox-b6, hoxb6, zf22

货号: IPDX32752

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/1000	zebrafish
IF	咨询技术	zebrafish
IHC	咨询技术	zebrafish
ICC	技术咨询	zebrafish
FCM	咨询技术	zebrafish
Elisa	咨询技术	zebrafish

产品详情

Aliases	Homeobox protein Hox-B6a, Hox-B6, Homeobox protein Zf-22, hoxb6a, hox-b6, hoxb6, zf22
Entrez GeneID	30341
WB Predicted band size	26.1kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	zebrafish
Immunogen	This (DANRE) hoxb6a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 98-131 amino acids of DANRE hoxb6a.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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Western blot analysis of lysate from zebra fish muscle tissue lysate, using (DANRE) hoxb6a Antibody (Center)(Cat. #P32752). P32752 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

参考文献

以下是模拟的参考文献示例(注：以下为虚构内容，建议通过PubMed、Google Scholar等平台查询真实文献)：

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1. **"Spatiotemporal expression of hoxb6a in zebrafish embryogenesis"**

*Author: Müller F, et al.*

摘要：通过hoxb6a特异性抗体免疫组化分析，揭示了该基因在斑马鱼后部体节和尾芽发育中的动态表达模式，提示其参与体轴分节调控。

2. **"Hoxb6a regulates fin patterning via Wnt signaling in Danio rerio"**

*Author: Tanaka H, et al.*

摘要：利用hoxb6a抗体进行蛋白定位及功能缺失实验，证明其通过调控Wnt通路影响斑马鱼胸鳍形态发生，揭示进化保守的Hox基因功能。

3. **"Antibody validation for zebrafish Hox proteins: A case study of hoxb6a"**

*Author: Patel R, et al.*

摘要：系统评估了hoxb6a抗体的特异性(Western Blot/免疫荧光)，证实其在斑马鱼胚胎及幼体中的可靠应用，为后续研究提供实验依据。

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**建议检索关键词**：

- "zebrafish hoxb6a antibody"

- "hoxb6a Danio rerio immunohistochemistry"

- "hoxb6a function development"

真实文献可通过以上关键词在学术数据库中定位。

背景信息

The DANRE (Danio rerio) Hoxb6a antibody is a specialized tool used in developmental biology to study the spatial and temporal expression patterns of the Hoxb6a protein in zebrafish. Hox genes, including Hoxb6a, encode homeodomain transcription factors critical for establishing body axis segmentation and regional identity during embryogenesis. In zebrafish, Hoxb6a is implicated in patterning the posterior trunk and tail regions, particularly influencing fin development and somite formation.

This antibody is typically raised against conserved epitopes of the Hoxb6a protein, often in rabbit or mouse hosts, and validated for specificity through techniques like Western blotting, immunohistochemistry (IHC), or whole-mount in situ hybridization (WISH). It enables researchers to visualize Hoxb6a expression in zebrafish embryos, aiding investigations into gene regulation, evolutionary conservation of Hox clusters, and mechanisms underlying axial patterning defects.

Studies using the DANRE Hoxb6a antibody contribute to understanding how Hox genes orchestrate tissue-specific differentiation and morphogenesis. Its applications extend to comparative genomics, toxicology (assessing developmental disruptions), and regenerative medicine. Proper controls, such as knockout models or peptide competition assays, are essential to confirm antibody specificity in experimental setups.